Synthesis of glycerol nucleic acid (GNA) phosphoramidite monomers and oligonucleotide polymers

Su Zhang, John C. Chaput

Research output: Contribution to journalReview articlepeer-review

2 Scopus citations

Abstract

This unit describes a straightforward method for preparing glycerol nucleic acid (GNA) phosphoramidite monomers and oligonucleotide polymers using standard cyanoethyl phosphoramidite chemistry. GNA is an unnatural nucleic acid analog composed of an acyclic three-carbon sugar-phosphate backbone that contains one stereogenic center per repeating unit. GNA has attracted significant attention as a nucleic acid derivative due to its unique ability to form stable Watson-Crick anti-parallel duplex structures with thermal and thermodynamic stabilities rivaling those of natural DNA and RNA. The chemical simplicity of this nucleic acid structure provides access to enantiomerically pure forms of right- and left-handed helical structures that can be used as unnatural building blocks in DNA nanotechnology.

Original languageEnglish (US)
Pages (from-to)4.40.1-4.40.18
JournalCurrent Protocols in Nucleic Acid Chemistry
Issue numberSUPPL. 42
DOIs
StatePublished - Sep 28 2010

Keywords

  • chemical synthesis
  • glycerol nucleic acid (GNA)
  • nanotechnology
  • oligonucleotide
  • phosphoramidite
  • solid-phase synthesis
  • thermal stability

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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