Synthesis of cyanobacterial RNA-binding proteins is regulated by cold- and osmotic-stress

S. Mori, D. Römern, Peter Lammers

Research output: Contribution to journalArticle

Abstract

Adaptation to cold stress in bacteria and plants ran be critical for longterm .survival, and represents a significant challenge to agriculture. It is known that plant;, pre-adapted to dehydration or osmotic stress exhibit enhanced cold tolérance. However, little is known about cross-protection between cold and dehydration stresses in bacterial systems. We study mechanisms of desiccation tolerance in the cyanobacterium Anabaena P('( 7120. Anabaena 7120 contains four R.\A binding proteins encoded bv the rbpA.B.C and I) genes. We show that expression of the RbpH protein in Anabaena 7120 is stimulated by cold stress, osmotic stress, salt stress and inatric water slress. High stringency Northern hybridizations with an rbplî probe show that transcripts accumulate to similar high levels after cold shift (30 to 20 C) and osmotic stress with 250 ni.M sucrose. Treatment with 60 m.\I NaCl and overnight desiccation also caused rbpH transcript accuimilation bul to a. significantly lower extent. Western blot.s probed confirm that total Rbp protein levels change in response to both cold and osmotic stresses. Treatments that increase Rbp protein expression also r n h a n ce survival of this si rain during severe cold stress. Cultures of Anabaena 7) 20 adapted to osmotic stress prior to cryogenic storage had four-fold higher revival rates that untreated controls. Furthermore, successive pre-treatments with osmotic stress then chilling stress increased viable cell counts after cryogenic storage by iH fold over non-treated controls. The RNA binding proteins in Anabaena 7120 may act as RNA chaparones during both cold and dehydration -tirxM'-. .1- proposed for the (Vp \protein f'oni K.s< herchia roli.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number8
StatePublished - 1998
Externally publishedYes

Fingerprint

RNA-binding proteins
RNA-Binding Proteins
Osmotic Pressure
cold stress
osmotic stress
Anabaena
synthesis
Dehydration
Desiccation
Cryogenics
Proteins
protein synthesis
Chilling
Cross Protection
Northern blotting
Agriculture
probes (equipment)
salt stress
Rain
Sucrose

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Synthesis of cyanobacterial RNA-binding proteins is regulated by cold- and osmotic-stress. / Mori, S.; Römern, D.; Lammers, Peter.

In: FASEB Journal, Vol. 12, No. 8, 1998.

Research output: Contribution to journalArticle

@article{cc50537ea6374ebead95e77ca303e7c2,
title = "Synthesis of cyanobacterial RNA-binding proteins is regulated by cold- and osmotic-stress",
abstract = "Adaptation to cold stress in bacteria and plants ran be critical for longterm .survival, and represents a significant challenge to agriculture. It is known that plant;, pre-adapted to dehydration or osmotic stress exhibit enhanced cold tol{\'e}rance. However, little is known about cross-protection between cold and dehydration stresses in bacterial systems. We study mechanisms of desiccation tolerance in the cyanobacterium Anabaena P('( 7120. Anabaena 7120 contains four R.\A binding proteins encoded bv the rbpA.B.C and I) genes. We show that expression of the RbpH protein in Anabaena 7120 is stimulated by cold stress, osmotic stress, salt stress and inatric water slress. High stringency Northern hybridizations with an rbpl{\^i} probe show that transcripts accumulate to similar high levels after cold shift (30 to 20 C) and osmotic stress with 250 ni.M sucrose. Treatment with 60 m.\I NaCl and overnight desiccation also caused rbpH transcript accuimilation bul to a. significantly lower extent. Western blot.s probed confirm that total Rbp protein levels change in response to both cold and osmotic stresses. Treatments that increase Rbp protein expression also r n h a n ce survival of this si rain during severe cold stress. Cultures of Anabaena 7) 20 adapted to osmotic stress prior to cryogenic storage had four-fold higher revival rates that untreated controls. Furthermore, successive pre-treatments with osmotic stress then chilling stress increased viable cell counts after cryogenic storage by iH fold over non-treated controls. The RNA binding proteins in Anabaena 7120 may act as RNA chaparones during both cold and dehydration -tirxM'-. .1- proposed for the (Vp \protein f'oni K.s< herchia roli.",
author = "S. Mori and D. R{\"o}mern and Peter Lammers",
year = "1998",
language = "English (US)",
volume = "12",
journal = "The FASEB journal : official publication of the Federation of American Societies for Experimental Biology",
issn = "0892-6638",
publisher = "FASEB",
number = "8",

}

TY - JOUR

T1 - Synthesis of cyanobacterial RNA-binding proteins is regulated by cold- and osmotic-stress

AU - Mori, S.

AU - Römern, D.

AU - Lammers, Peter

PY - 1998

Y1 - 1998

N2 - Adaptation to cold stress in bacteria and plants ran be critical for longterm .survival, and represents a significant challenge to agriculture. It is known that plant;, pre-adapted to dehydration or osmotic stress exhibit enhanced cold tolérance. However, little is known about cross-protection between cold and dehydration stresses in bacterial systems. We study mechanisms of desiccation tolerance in the cyanobacterium Anabaena P('( 7120. Anabaena 7120 contains four R.\A binding proteins encoded bv the rbpA.B.C and I) genes. We show that expression of the RbpH protein in Anabaena 7120 is stimulated by cold stress, osmotic stress, salt stress and inatric water slress. High stringency Northern hybridizations with an rbplî probe show that transcripts accumulate to similar high levels after cold shift (30 to 20 C) and osmotic stress with 250 ni.M sucrose. Treatment with 60 m.\I NaCl and overnight desiccation also caused rbpH transcript accuimilation bul to a. significantly lower extent. Western blot.s probed confirm that total Rbp protein levels change in response to both cold and osmotic stresses. Treatments that increase Rbp protein expression also r n h a n ce survival of this si rain during severe cold stress. Cultures of Anabaena 7) 20 adapted to osmotic stress prior to cryogenic storage had four-fold higher revival rates that untreated controls. Furthermore, successive pre-treatments with osmotic stress then chilling stress increased viable cell counts after cryogenic storage by iH fold over non-treated controls. The RNA binding proteins in Anabaena 7120 may act as RNA chaparones during both cold and dehydration -tirxM'-. .1- proposed for the (Vp \protein f'oni K.s< herchia roli.

AB - Adaptation to cold stress in bacteria and plants ran be critical for longterm .survival, and represents a significant challenge to agriculture. It is known that plant;, pre-adapted to dehydration or osmotic stress exhibit enhanced cold tolérance. However, little is known about cross-protection between cold and dehydration stresses in bacterial systems. We study mechanisms of desiccation tolerance in the cyanobacterium Anabaena P('( 7120. Anabaena 7120 contains four R.\A binding proteins encoded bv the rbpA.B.C and I) genes. We show that expression of the RbpH protein in Anabaena 7120 is stimulated by cold stress, osmotic stress, salt stress and inatric water slress. High stringency Northern hybridizations with an rbplî probe show that transcripts accumulate to similar high levels after cold shift (30 to 20 C) and osmotic stress with 250 ni.M sucrose. Treatment with 60 m.\I NaCl and overnight desiccation also caused rbpH transcript accuimilation bul to a. significantly lower extent. Western blot.s probed confirm that total Rbp protein levels change in response to both cold and osmotic stresses. Treatments that increase Rbp protein expression also r n h a n ce survival of this si rain during severe cold stress. Cultures of Anabaena 7) 20 adapted to osmotic stress prior to cryogenic storage had four-fold higher revival rates that untreated controls. Furthermore, successive pre-treatments with osmotic stress then chilling stress increased viable cell counts after cryogenic storage by iH fold over non-treated controls. The RNA binding proteins in Anabaena 7120 may act as RNA chaparones during both cold and dehydration -tirxM'-. .1- proposed for the (Vp \protein f'oni K.s< herchia roli.

UR - http://www.scopus.com/inward/record.url?scp=33749113657&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749113657&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749113657

VL - 12

JO - The FASEB journal : official publication of the Federation of American Societies for Experimental Biology

JF - The FASEB journal : official publication of the Federation of American Societies for Experimental Biology

SN - 0892-6638

IS - 8

ER -