Synthesis of cationic polymer libraries for gene delivery using diglycidyl ethers

Jacob Elmer, Thrimoorthy Potta, Kaushal Rege

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Gene therapy has the potential to cure many different genetic diseases, if safe and effective gene delivery vectors can be developed. This chapter describes protocols for the synthesis of novel polymers using diglycidyl ether and diamine or polyamine monomers for transgene delivery and expression. The resulting poly (amino ethers) are able to transfect a higher number of cells, with lower cytotoxicity than other commercially available polymers (e.g., Polyethyleneimine, PEI).

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages3-16
Number of pages14
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1831
ISSN (Print)1064-3745

Fingerprint

Ethers
Gene Library
Polymers
Polyethyleneimine
Inborn Genetic Diseases
Diamines
Polyamines
Transgenes
Genetic Therapy
Ether
Cell Count
Genes

Keywords

  • Aminoglycosides
  • Diglycidyl ethers
  • Gene delivery
  • Gene therapy
  • Polymers
  • Polyplex

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Elmer, J., Potta, T., & Rege, K. (2018). Synthesis of cationic polymer libraries for gene delivery using diglycidyl ethers. In Methods in Molecular Biology (pp. 3-16). (Methods in Molecular Biology; Vol. 1831). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8661-3_1

Synthesis of cationic polymer libraries for gene delivery using diglycidyl ethers. / Elmer, Jacob; Potta, Thrimoorthy; Rege, Kaushal.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 3-16 (Methods in Molecular Biology; Vol. 1831).

Research output: Chapter in Book/Report/Conference proceedingChapter

Elmer, J, Potta, T & Rege, K 2018, Synthesis of cationic polymer libraries for gene delivery using diglycidyl ethers. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1831, Humana Press Inc., pp. 3-16. https://doi.org/10.1007/978-1-4939-8661-3_1
Elmer J, Potta T, Rege K. Synthesis of cationic polymer libraries for gene delivery using diglycidyl ethers. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 3-16. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8661-3_1
Elmer, Jacob ; Potta, Thrimoorthy ; Rege, Kaushal. / Synthesis of cationic polymer libraries for gene delivery using diglycidyl ethers. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 3-16 (Methods in Molecular Biology).
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