Synthesis in Escherichia coli of the major glycoprotein of human rotavirus

analysis of the antigenic regions

M. A. Johnson, Rajeev Misra, M. Lardelli, M. Messina, C. Ephraums, P. R. Reeves, Z. Bolcevic, J. S. Noel, C. P. Hum, H. Van Mai, M. L. Dyall-Smith, I. H. Holmes

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Various regions of the gene encoding the major neutralization antigen, VP7, of human rotavirus have been expressed in Escherichia coli, as N-terminal fusions to β-galactosidase under the control of the lac promoter. We have determined that the fusion products of two clones containing regions AB (aa 69-158) and ABC (aa 69-319) were antigenic, reacting with antibodies raised against whole virus. When guinea pigs were immunized with fusion protein purified by monoclonal antibody affinity columns, no neutralizing or virus-binding anti-bodies were detected, but antibodies binding to denatured VP7 were present.

Original languageEnglish (US)
Pages (from-to)73-81
Number of pages9
JournalGene
Volume84
Issue number1
DOIs
StatePublished - Dec 7 1989
Externally publishedYes

Fingerprint

Rotavirus
Glycoproteins
Galactosidases
Escherichia coli
Virus Attachment
Antibody Affinity
Antibodies
Guinea Pigs
Clone Cells
Monoclonal Antibodies
Viruses
Antigens
Genes
Proteins

Keywords

  • fusion protein
  • gene cloning
  • Recombinant DNA
  • viral vaccines
  • Western blotting

ASJC Scopus subject areas

  • Genetics

Cite this

Johnson, M. A., Misra, R., Lardelli, M., Messina, M., Ephraums, C., Reeves, P. R., ... Holmes, I. H. (1989). Synthesis in Escherichia coli of the major glycoprotein of human rotavirus: analysis of the antigenic regions. Gene, 84(1), 73-81. https://doi.org/10.1016/0378-1119(89)90141-8

Synthesis in Escherichia coli of the major glycoprotein of human rotavirus : analysis of the antigenic regions. / Johnson, M. A.; Misra, Rajeev; Lardelli, M.; Messina, M.; Ephraums, C.; Reeves, P. R.; Bolcevic, Z.; Noel, J. S.; Hum, C. P.; Van Mai, H.; Dyall-Smith, M. L.; Holmes, I. H.

In: Gene, Vol. 84, No. 1, 07.12.1989, p. 73-81.

Research output: Contribution to journalArticle

Johnson, MA, Misra, R, Lardelli, M, Messina, M, Ephraums, C, Reeves, PR, Bolcevic, Z, Noel, JS, Hum, CP, Van Mai, H, Dyall-Smith, ML & Holmes, IH 1989, 'Synthesis in Escherichia coli of the major glycoprotein of human rotavirus: analysis of the antigenic regions', Gene, vol. 84, no. 1, pp. 73-81. https://doi.org/10.1016/0378-1119(89)90141-8
Johnson, M. A. ; Misra, Rajeev ; Lardelli, M. ; Messina, M. ; Ephraums, C. ; Reeves, P. R. ; Bolcevic, Z. ; Noel, J. S. ; Hum, C. P. ; Van Mai, H. ; Dyall-Smith, M. L. ; Holmes, I. H. / Synthesis in Escherichia coli of the major glycoprotein of human rotavirus : analysis of the antigenic regions. In: Gene. 1989 ; Vol. 84, No. 1. pp. 73-81.
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