Abstract
Various regions of the gene encoding the major neutralization antigen, VP7, of human rotavirus have been expressed in Escherichia coli, as N-terminal fusions to β-galactosidase under the control of the lac promoter. We have determined that the fusion products of two clones containing regions AB (aa 69-158) and ABC (aa 69-319) were antigenic, reacting with antibodies raised against whole virus. When guinea pigs were immunized with fusion protein purified by monoclonal antibody affinity columns, no neutralizing or virus-binding anti-bodies were detected, but antibodies binding to denatured VP7 were present.
Original language | English (US) |
---|---|
Pages (from-to) | 73-81 |
Number of pages | 9 |
Journal | Gene |
Volume | 84 |
Issue number | 1 |
DOIs | |
State | Published - Dec 7 1989 |
Externally published | Yes |
Keywords
- Recombinant DNA
- Western blotting
- fusion protein
- gene cloning
- viral vaccines
ASJC Scopus subject areas
- Genetics