Synthesis in Escherichia coli of the major glycoprotein of human rotavirus: analysis of the antigenic regions

M. A. Johnson, R. M. Misra, M. Lardelli, M. Messina, C. Ephraums, P. R. Reeves, Z. Bolcevic, J. S. Noel, C. P. Hum, H. Van Mai, M. L. Dyall-Smith, I. H. Holmes

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Various regions of the gene encoding the major neutralization antigen, VP7, of human rotavirus have been expressed in Escherichia coli, as N-terminal fusions to β-galactosidase under the control of the lac promoter. We have determined that the fusion products of two clones containing regions AB (aa 69-158) and ABC (aa 69-319) were antigenic, reacting with antibodies raised against whole virus. When guinea pigs were immunized with fusion protein purified by monoclonal antibody affinity columns, no neutralizing or virus-binding anti-bodies were detected, but antibodies binding to denatured VP7 were present.

Original languageEnglish (US)
Pages (from-to)73-81
Number of pages9
JournalGene
Volume84
Issue number1
DOIs
StatePublished - Dec 7 1989
Externally publishedYes

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Keywords

  • Recombinant DNA
  • Western blotting
  • fusion protein
  • gene cloning
  • viral vaccines

ASJC Scopus subject areas

  • Genetics

Cite this

Johnson, M. A., Misra, R. M., Lardelli, M., Messina, M., Ephraums, C., Reeves, P. R., Bolcevic, Z., Noel, J. S., Hum, C. P., Van Mai, H., Dyall-Smith, M. L., & Holmes, I. H. (1989). Synthesis in Escherichia coli of the major glycoprotein of human rotavirus: analysis of the antigenic regions. Gene, 84(1), 73-81. https://doi.org/10.1016/0378-1119(89)90141-8