Synthesis, Cleavage, and Binding Disruption Using Polymer Microstructures as Virtual Flasks for Photoacids/Photobases

Neal Woodbury (Inventor)

Research output: Patent

Abstract

Currently, the stepwise yield for photolabile groups used to synthesize arrays have less than quantitative yields. We hope hat this method may be the way to improve the stepwise yield for deprotection which would enable the contruction of the long peptides or other heteropolymers.This also provides a method for using lieght to selectively remove materials from the array, in the case of materials bound to the array, an acid labile linker can be used to attach the heteropolymer to the micurostructure. Upon illumination of the photoacid/base or buffer solution the heteropolymer can be slectively removed from one or more microstructures and characterized using a common analytical method such as mass spectrometry.In the case of protein or other materials bound to heteropolymer grafted microstructures, the same photoacid buffer or base system can be used to selectively disrupt or disrupt the interactions between the heteropolymer and the protein such that the protein can be selectively removed from microstructures of interest. These can then be characterized using common analytical techniques such as mass spectrometry. This would enable large arrays to be screen for proteins binding using fluorescence and then the bound proteins to be characterized. It would also be possible to identify multiple protein bound to a single mircrostructure using this technology.
Original languageEnglish (US)
Publication statusPublished - Jan 27 2006

    Fingerprint

Cite this