Synthesis and turnover of photosystem II reaction center polypeptides in cyanobacterial D2 mutants

Jiujiang Yu, Willem Vermaas

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Several mutations in psbD (coding for the photosystem II reaction protein D2) lead to a loss of the entire photosystem II complex from the thylakoid membrane. For two site-directed D2 mutants, E69V (Glu-69 mutated to Val) and ET-9 (introducing a premature stop codon in psbD), and two psbD deletion mutants, the synthesis and degradation of photosystem II reaction center components were analyzed. In E69V, no D2 or psbC-encoded CP43 was detected upon 5-min pulse labeling or Western blotting, whereas a psbDI/C transcript was present. Either D2 and CP43 are very unstable in this mutant, or the mutation causes an inhibition of translation of the psbDC operon. In E69V, the predominant lifetime of D1 is several minutes, but a small fraction of D1 remains stable in the thylakoid for several hours. In E69V, also a 43-kDa band is detected by D1 antisera. We attribute this band to a D1 cross-linking product possibly involved in D1 degradation. We identified a D1 precursor in E69V and psbDI/C/DII-, two mutants with particularly short D1 lifetimes. A unique feature of the E69V mutant is that destabilization of the photosystem II complex is triggered by a single-site mutation leading to extremely rapid turnover or lack of synthesis of D2.

Original languageEnglish (US)
Pages (from-to)7407-7413
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number10
StatePublished - Apr 5 1993

Fingerprint

Photosystem II Protein Complex
Peptides
Thylakoids
Mutation
Degradation
Nonsense Codon
Operon
Labeling
Immune Sera
Western Blotting
Membranes
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Synthesis and turnover of photosystem II reaction center polypeptides in cyanobacterial D2 mutants. / Yu, Jiujiang; Vermaas, Willem.

In: Journal of Biological Chemistry, Vol. 268, No. 10, 05.04.1993, p. 7407-7413.

Research output: Contribution to journalArticle

@article{d8958a640fdf4247bb870523324344c0,
title = "Synthesis and turnover of photosystem II reaction center polypeptides in cyanobacterial D2 mutants",
abstract = "Several mutations in psbD (coding for the photosystem II reaction protein D2) lead to a loss of the entire photosystem II complex from the thylakoid membrane. For two site-directed D2 mutants, E69V (Glu-69 mutated to Val) and ET-9 (introducing a premature stop codon in psbD), and two psbD deletion mutants, the synthesis and degradation of photosystem II reaction center components were analyzed. In E69V, no D2 or psbC-encoded CP43 was detected upon 5-min pulse labeling or Western blotting, whereas a psbDI/C transcript was present. Either D2 and CP43 are very unstable in this mutant, or the mutation causes an inhibition of translation of the psbDC operon. In E69V, the predominant lifetime of D1 is several minutes, but a small fraction of D1 remains stable in the thylakoid for several hours. In E69V, also a 43-kDa band is detected by D1 antisera. We attribute this band to a D1 cross-linking product possibly involved in D1 degradation. We identified a D1 precursor in E69V and psbDI/C/DII-, two mutants with particularly short D1 lifetimes. A unique feature of the E69V mutant is that destabilization of the photosystem II complex is triggered by a single-site mutation leading to extremely rapid turnover or lack of synthesis of D2.",
author = "Jiujiang Yu and Willem Vermaas",
year = "1993",
month = "4",
day = "5",
language = "English (US)",
volume = "268",
pages = "7407--7413",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "10",

}

TY - JOUR

T1 - Synthesis and turnover of photosystem II reaction center polypeptides in cyanobacterial D2 mutants

AU - Yu, Jiujiang

AU - Vermaas, Willem

PY - 1993/4/5

Y1 - 1993/4/5

N2 - Several mutations in psbD (coding for the photosystem II reaction protein D2) lead to a loss of the entire photosystem II complex from the thylakoid membrane. For two site-directed D2 mutants, E69V (Glu-69 mutated to Val) and ET-9 (introducing a premature stop codon in psbD), and two psbD deletion mutants, the synthesis and degradation of photosystem II reaction center components were analyzed. In E69V, no D2 or psbC-encoded CP43 was detected upon 5-min pulse labeling or Western blotting, whereas a psbDI/C transcript was present. Either D2 and CP43 are very unstable in this mutant, or the mutation causes an inhibition of translation of the psbDC operon. In E69V, the predominant lifetime of D1 is several minutes, but a small fraction of D1 remains stable in the thylakoid for several hours. In E69V, also a 43-kDa band is detected by D1 antisera. We attribute this band to a D1 cross-linking product possibly involved in D1 degradation. We identified a D1 precursor in E69V and psbDI/C/DII-, two mutants with particularly short D1 lifetimes. A unique feature of the E69V mutant is that destabilization of the photosystem II complex is triggered by a single-site mutation leading to extremely rapid turnover or lack of synthesis of D2.

AB - Several mutations in psbD (coding for the photosystem II reaction protein D2) lead to a loss of the entire photosystem II complex from the thylakoid membrane. For two site-directed D2 mutants, E69V (Glu-69 mutated to Val) and ET-9 (introducing a premature stop codon in psbD), and two psbD deletion mutants, the synthesis and degradation of photosystem II reaction center components were analyzed. In E69V, no D2 or psbC-encoded CP43 was detected upon 5-min pulse labeling or Western blotting, whereas a psbDI/C transcript was present. Either D2 and CP43 are very unstable in this mutant, or the mutation causes an inhibition of translation of the psbDC operon. In E69V, the predominant lifetime of D1 is several minutes, but a small fraction of D1 remains stable in the thylakoid for several hours. In E69V, also a 43-kDa band is detected by D1 antisera. We attribute this band to a D1 cross-linking product possibly involved in D1 degradation. We identified a D1 precursor in E69V and psbDI/C/DII-, two mutants with particularly short D1 lifetimes. A unique feature of the E69V mutant is that destabilization of the photosystem II complex is triggered by a single-site mutation leading to extremely rapid turnover or lack of synthesis of D2.

UR - http://www.scopus.com/inward/record.url?scp=0027531820&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027531820&partnerID=8YFLogxK

M3 - Article

VL - 268

SP - 7407

EP - 7413

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 10

ER -