Synthesis and enzymatic incorporation of α-l-threofuranosyl adenine triphosphate (tATP)

Su Zhang, John C. Chaput

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Threose nucleic acid (TNA) is an artificial genetic polymer in which the natural ribose sugar found in RNA has been replaced with an unnatural threose sugar. TNA can be synthesized enzymatically using Therminator DNA polymerase to copy DNA templates into TNA. Here, we expand the substrate repertoire of Therminator DNA polymerase to include threofuranosyl adenine 3′-triphsophate (tATP). We chemically synthesized tATP by two different methods from the 2′-O-acetyl derivative. Enzyme-mediated polymerization reveals that tATP functions as an efficient substrate for Therminator DNA polymerase, indicating that tATP can replace the diaminopurine analogue (tDTP) in TNA transcription reactions.

Original languageEnglish (US)
Pages (from-to)1447-1449
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume23
Issue number5
DOIs
StatePublished - Mar 1 2013

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Keywords

  • Synthetic genetics
  • Threose nucleic acid (TNA)

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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