Synthesis and enzymatic incorporation of α-l-threofuranosyl adenine triphosphate (tATP)

Su Zhang, John C. Chaput

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Threose nucleic acid (TNA) is an artificial genetic polymer in which the natural ribose sugar found in RNA has been replaced with an unnatural threose sugar. TNA can be synthesized enzymatically using Therminator DNA polymerase to copy DNA templates into TNA. Here, we expand the substrate repertoire of Therminator DNA polymerase to include threofuranosyl adenine 3′-triphsophate (tATP). We chemically synthesized tATP by two different methods from the 2′-O-acetyl derivative. Enzyme-mediated polymerization reveals that tATP functions as an efficient substrate for Therminator DNA polymerase, indicating that tATP can replace the diaminopurine analogue (tDTP) in TNA transcription reactions.

Original languageEnglish (US)
Pages (from-to)1447-1449
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume23
Issue number5
DOIs
StatePublished - Mar 1 2013

Fingerprint

Adenine
Nucleic Acids
DNA-Directed DNA Polymerase
Sugars
Ribose
Substrates
Transcription
Polymerization
Polymers
triphosphoric acid
erythrose
RNA
Derivatives
DNA
Enzymes

Keywords

  • Synthetic genetics
  • Threose nucleic acid (TNA)

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry
  • Biochemistry

Cite this

Synthesis and enzymatic incorporation of α-l-threofuranosyl adenine triphosphate (tATP). / Zhang, Su; Chaput, John C.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 23, No. 5, 01.03.2013, p. 1447-1449.

Research output: Contribution to journalArticle

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