Synthesis and characterization of peptide granted porous polymer microstructures

Trent R. Northen, Daniel C. Brune, Neal Woodbury

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A scanning laser system has been used to generate three-dimensional trimethylolpropane trimethacrylate (TRIM) cross-linked poly(2-hydroxylethyl methacrylate) polymer microstructures through azo-bis(isobutyro)nitrile (AIBN) photopolymerization using a 20 × 0.5 NA microscope objective and 365 nm laser excitation. Macropores are observed to form without the use of porogens in regions of highest light flux. This is attributed to phase separation, which results from differences in monomer reactivity and miscibility. The microstructures were aminated and then protected with the photolabile protective group 6-nitroveratryloxycarbonyl (NVOC). This made it possible to selectively modify the microstructures with the same scanning laser system that was used to fabricate them, resulting in peptide grafted three-dimensional porous microstructures. On the basis of the absorbance of the dibenzofulvene-piperidine, these structures have an amine site density of ∼0.1 nmol/feature. MALDI-TOF MS was used to characterize peptide photografted microstructures. N-Tris(2,4,6-trimethoxyphenyl)phosphonium (TMPP) labeling of the peptides greatly enhanced detection and allowed post-source decay sequencing of the peptides from the microstructures. The techniques described could be used to generate three-dimensional peptide grafted porous scaffolds for tissue engineering applications.

Original languageEnglish (US)
Pages (from-to)750-754
Number of pages5
JournalBiomacromolecules
Volume7
Issue number3
DOIs
StatePublished - Mar 2006

Fingerprint

Peptides
Polymers
Microstructure
Lasers
Nitriles
Methacrylates
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Scanning
Tissue Engineering
Laser excitation
Photopolymerization
Amines
Scaffolds (biology)
Tissue engineering
Phase separation
Labeling
Microscopes
Light
Solubility
Monomers

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Synthesis and characterization of peptide granted porous polymer microstructures. / Northen, Trent R.; Brune, Daniel C.; Woodbury, Neal.

In: Biomacromolecules, Vol. 7, No. 3, 03.2006, p. 750-754.

Research output: Contribution to journalArticle

Northen, Trent R. ; Brune, Daniel C. ; Woodbury, Neal. / Synthesis and characterization of peptide granted porous polymer microstructures. In: Biomacromolecules. 2006 ; Vol. 7, No. 3. pp. 750-754.
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