Synbodies for Detection of Human Norovirus

Charles Arntzen (Inventor), Stephen Johnston (Inventor)

Research output: Patent

Abstract

Noroviruses are hardy, highly contagious viruses that are the most common cause of acute gastroenteritis in humans. The CDC estimates that there are about 21 million illnesses caused by Noroviruses each year and about 800 of those result in death. There is no available vaccine for Norovirus infection and vaccine progress is hampered by the absence of suitable animal model/cell culture systems for preclinical testing of the candidate vaccine. Additionally, detection of viral RNA is limited to RT-PCR of stool samples. Researchers at the Biodesign Institute of Arizona State University have developed novel synthetic antibodies (synbodies) with high affinity toward noroviruses. These synbodies bind to the geno-groups of Norovirus that are known to infect humans, namely GI, and GII. Moreover, these synbodies function in ELISA, Western Blot, and pull-down assays. These novel synbodies and simple ELISA detection method provide an easy and sensitive detection means for GI and GII Norovirus strains and may also help enable the ultimate production of a Norovirus vaccine. Potential Applications Norovirus detection platform The synbodies can be coupled with filtration procedures to evaluate candidate non-clinical sample matrices (food, water and environment) Removal of low levels of viruses present in naturally contaminated samples Benefits and Advantages High sensitivity and specificity Used as detection or enrichment agents for Norovirus detection Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. Diehnelt's directory webpage Dr. Arntzen's directory webpage Dr. Arntzen's departmental webpage Dr. Johnston's directory webpage
Original languageEnglish (US)
StatePublished - Sep 14 2012

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Norovirus
Directories
Vaccines
Antibodies
Enzyme-Linked Immunosorbent Assay
Inventors
Viruses
Viral RNA
Gastroenteritis
Centers for Disease Control and Prevention (U.S.)
Animal Models
Cell Culture Techniques
Western Blotting
Research Personnel
Sensitivity and Specificity
Food
Polymerase Chain Reaction

Cite this

Synbodies for Detection of Human Norovirus. / Arntzen, Charles (Inventor); Johnston, Stephen (Inventor).

Research output: Patent

Arntzen C, Johnston S, inventors. Synbodies for Detection of Human Norovirus. 2012 Sep 14.
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abstract = "Noroviruses are hardy, highly contagious viruses that are the most common cause of acute gastroenteritis in humans. The CDC estimates that there are about 21 million illnesses caused by Noroviruses each year and about 800 of those result in death. There is no available vaccine for Norovirus infection and vaccine progress is hampered by the absence of suitable animal model/cell culture systems for preclinical testing of the candidate vaccine. Additionally, detection of viral RNA is limited to RT-PCR of stool samples. Researchers at the Biodesign Institute of Arizona State University have developed novel synthetic antibodies (synbodies) with high affinity toward noroviruses. These synbodies bind to the geno-groups of Norovirus that are known to infect humans, namely GI, and GII. Moreover, these synbodies function in ELISA, Western Blot, and pull-down assays. These novel synbodies and simple ELISA detection method provide an easy and sensitive detection means for GI and GII Norovirus strains and may also help enable the ultimate production of a Norovirus vaccine. Potential Applications Norovirus detection platform The synbodies can be coupled with filtration procedures to evaluate candidate non-clinical sample matrices (food, water and environment) Removal of low levels of viruses present in naturally contaminated samples Benefits and Advantages High sensitivity and specificity Used as detection or enrichment agents for Norovirus detection Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. Diehnelt's directory webpage Dr. Arntzen's directory webpage Dr. Arntzen's departmental webpage Dr. Johnston's directory webpage",
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N2 - Noroviruses are hardy, highly contagious viruses that are the most common cause of acute gastroenteritis in humans. The CDC estimates that there are about 21 million illnesses caused by Noroviruses each year and about 800 of those result in death. There is no available vaccine for Norovirus infection and vaccine progress is hampered by the absence of suitable animal model/cell culture systems for preclinical testing of the candidate vaccine. Additionally, detection of viral RNA is limited to RT-PCR of stool samples. Researchers at the Biodesign Institute of Arizona State University have developed novel synthetic antibodies (synbodies) with high affinity toward noroviruses. These synbodies bind to the geno-groups of Norovirus that are known to infect humans, namely GI, and GII. Moreover, these synbodies function in ELISA, Western Blot, and pull-down assays. These novel synbodies and simple ELISA detection method provide an easy and sensitive detection means for GI and GII Norovirus strains and may also help enable the ultimate production of a Norovirus vaccine. Potential Applications Norovirus detection platform The synbodies can be coupled with filtration procedures to evaluate candidate non-clinical sample matrices (food, water and environment) Removal of low levels of viruses present in naturally contaminated samples Benefits and Advantages High sensitivity and specificity Used as detection or enrichment agents for Norovirus detection Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. Diehnelt's directory webpage Dr. Arntzen's directory webpage Dr. Arntzen's departmental webpage Dr. Johnston's directory webpage

AB - Noroviruses are hardy, highly contagious viruses that are the most common cause of acute gastroenteritis in humans. The CDC estimates that there are about 21 million illnesses caused by Noroviruses each year and about 800 of those result in death. There is no available vaccine for Norovirus infection and vaccine progress is hampered by the absence of suitable animal model/cell culture systems for preclinical testing of the candidate vaccine. Additionally, detection of viral RNA is limited to RT-PCR of stool samples. Researchers at the Biodesign Institute of Arizona State University have developed novel synthetic antibodies (synbodies) with high affinity toward noroviruses. These synbodies bind to the geno-groups of Norovirus that are known to infect humans, namely GI, and GII. Moreover, these synbodies function in ELISA, Western Blot, and pull-down assays. These novel synbodies and simple ELISA detection method provide an easy and sensitive detection means for GI and GII Norovirus strains and may also help enable the ultimate production of a Norovirus vaccine. Potential Applications Norovirus detection platform The synbodies can be coupled with filtration procedures to evaluate candidate non-clinical sample matrices (food, water and environment) Removal of low levels of viruses present in naturally contaminated samples Benefits and Advantages High sensitivity and specificity Used as detection or enrichment agents for Norovirus detection Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. Diehnelt's directory webpage Dr. Arntzen's directory webpage Dr. Arntzen's departmental webpage Dr. Johnston's directory webpage

M3 - Patent

ER -