Swimming of Xenopus laevis sperm exhibits multiple gears and its duration is extended by egg jelly constituents

Nathan Tholl, Sumera Naqvi, Ericka McLaughlin, Serenity Boyles, Allan L. Bieber, Douglas E. Chandler

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The motility of Xenopus sperm is initiated by the osmotic shock experienced when these cells are ejaculated into low-salinity pond water. Motility is brief and is required for the sperm to penetrate the jelly layers and fertilize the egg. In this study we demonstrate that extracts of egg jelly contain factors that extend the period of sperm motility as well as providing a chemoattractant activity as previously reported. Both activities are partially dependent on extracellular calcium. Time-lapse and video microscopy show that after activation of motility the number of motile sperm decreases rapidly, with a half-time of about 2 min. Addition of 10% v/v egg jelly extract ("egg water") increased the number of motile sperm 2-fold over controls at 20 s and about 4- to 10-fold over controls at 10 min after initiation of motility. Extension of motility lifetime was not mediated by a nonspecific protein or by allurin, the eggwater protein that has chemoattractant activity. The helical path of Xenopus sperm exhibited tight coupling between rotational and forward velocities in egg jelly, but coupling changed rapidly from moment to moment in low-salinity buffer. Our observations suggest that jelly-derived factors regulate both the longevity and directionality of sperm propulsion.

Original languageEnglish (US)
Pages (from-to)174-185
Number of pages12
JournalBiological Bulletin
Volume220
Issue number3
DOIs
StatePublished - Jan 1 2011

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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    Tholl, N., Naqvi, S., McLaughlin, E., Boyles, S., Bieber, A. L., & Chandler, D. E. (2011). Swimming of Xenopus laevis sperm exhibits multiple gears and its duration is extended by egg jelly constituents. Biological Bulletin, 220(3), 174-185. https://doi.org/10.1086/BBLv220n3p174