Susceptibility and protective mechanisms of motile and non motile cells of haematococcus pluvialis (chlorophyceae) to photooxidative stress

Danxiang Han, Junfeng Wang, Milton Sommerfeld, Qiang Hu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The life cycle of the unicellular green alga Haematococcus pluvialis consists of motile and nonmotile stages under typical growing conditions. In this study, we observed that motile cells were more susceptible than nonmotile cells to high light, resulting in a decrease in population density and photo-bleaching. Using two Haematococcus strains, CCAP 34/12 (a motile cell dominated strain) and SAG 34/1b (a nonmotile cell dominated strain), as model systems we investigated the cause of cell death and the protective mechanisms of the cells that survived high light. The death of motile cells under high light was attributed to the generation of excess reactive oxygen species (ROS), which caused severe damage to the photosynthetic components and the membrane system. Motile cells were able to dissipate excess light energy by nonphotochemical quenching and to relax ROS production by a partially up-regulated scavenging enzyme system. However, these strategies were not sufficient to protect the motile cells from high light stress. In contrast, nonmotile cells were able to cope with and survive under high light by (i) relaxing the over-reduced photosynthetic electron transport chain (PETC), thereby effectively utilizing PETC-generated NADPH to produce storage starch, neutral lipid, and astaxanthin, and thus preventing formation of excess ROS; (ii) down-regulating the linear electron transport by decreasing the level of cytochrome f; and (iii) consuming excess electrons produced by PSII via a significantly enhanced plastid terminal oxidase pathway.

Original languageEnglish (US)
Pages (from-to)693-705
Number of pages13
JournalJournal of Phycology
Volume48
Issue number3
DOIs
StatePublished - Jun 2012

Fingerprint

Haematococcus pluvialis
Chlorophyceae
electron
cells
reactive oxygen species
electron transport chain
cell death
Haematococcus
plastid
cytochrome f
bleaching
green alga
starch
photobleaching
cytochrome
astaxanthin
population density
life cycle
lipid
NADP (coenzyme)

Keywords

  • Haematococcus pluvialis
  • Motile cell
  • Nonmotile cell
  • Photooxidative stress

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Susceptibility and protective mechanisms of motile and non motile cells of haematococcus pluvialis (chlorophyceae) to photooxidative stress. / Han, Danxiang; Wang, Junfeng; Sommerfeld, Milton; Hu, Qiang.

In: Journal of Phycology, Vol. 48, No. 3, 06.2012, p. 693-705.

Research output: Contribution to journalArticle

Han, Danxiang ; Wang, Junfeng ; Sommerfeld, Milton ; Hu, Qiang. / Susceptibility and protective mechanisms of motile and non motile cells of haematococcus pluvialis (chlorophyceae) to photooxidative stress. In: Journal of Phycology. 2012 ; Vol. 48, No. 3. pp. 693-705.
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