SURVEY AND SUMMARY the structural diversity of artificial genetic polymers

Irina Anosova, Ewa A. Kowal, Matthew R. Dunn, John C. Chaput, Wade Van Horn, Martin Egli

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Synthetic genetics is a subdiscipline of synthetic biology that aims to develop artificial genetic polymers (also referred to as xeno-nucleic acids or XNAs) that can replicate in vitro and eventually in model cellular organisms. This field of science combines organic chemistry with polymerase engineering to create alternative forms of DNA that can store genetic information and evolve in response to external stimuli. Practitioners of synthetic genetics postulate that XNA could be used to safeguard synthetic biology organisms by storing genetic information in orthogonal chromosomes. XNA polymers are also under active investigation as a source of nuclease resistant affinity reagents (aptamers) and catalysts (xenozymes) with practical applications in disease diagnosis and treatment. In this review, we provide a structural perspective on known antiparallel duplex structures in which at least one strand of the Watson-Crick duplex is composed entirely of XNA. Currently, only a handful of XNA structures have been archived in the Protein Data Bank as compared to the more than 100 000 structures that are now available. Given the growing interest in xenobiology projects, we chose to compare the structural features of XNA polymers and discuss their potential to access new regions of nucleic acid fold space.

Original languageEnglish (US)
Pages (from-to)1007-1021
Number of pages15
JournalNucleic Acids Research
Volume44
Issue number3
DOIs
StatePublished - 2015

Fingerprint

Synthetic Biology
Polymers
Nucleic Acids
Organic Chemistry
Chromosomes
Databases
DNA
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics

Cite this

SURVEY AND SUMMARY the structural diversity of artificial genetic polymers. / Anosova, Irina; Kowal, Ewa A.; Dunn, Matthew R.; Chaput, John C.; Van Horn, Wade; Egli, Martin.

In: Nucleic Acids Research, Vol. 44, No. 3, 2015, p. 1007-1021.

Research output: Contribution to journalArticle

Anosova, I, Kowal, EA, Dunn, MR, Chaput, JC, Van Horn, W & Egli, M 2015, 'SURVEY AND SUMMARY the structural diversity of artificial genetic polymers', Nucleic Acids Research, vol. 44, no. 3, pp. 1007-1021. https://doi.org/10.1093/nar/gkv1472
Anosova, Irina ; Kowal, Ewa A. ; Dunn, Matthew R. ; Chaput, John C. ; Van Horn, Wade ; Egli, Martin. / SURVEY AND SUMMARY the structural diversity of artificial genetic polymers. In: Nucleic Acids Research. 2015 ; Vol. 44, No. 3. pp. 1007-1021.
@article{f9c6060318eb47e881b8194dde191e39,
title = "SURVEY AND SUMMARY the structural diversity of artificial genetic polymers",
abstract = "Synthetic genetics is a subdiscipline of synthetic biology that aims to develop artificial genetic polymers (also referred to as xeno-nucleic acids or XNAs) that can replicate in vitro and eventually in model cellular organisms. This field of science combines organic chemistry with polymerase engineering to create alternative forms of DNA that can store genetic information and evolve in response to external stimuli. Practitioners of synthetic genetics postulate that XNA could be used to safeguard synthetic biology organisms by storing genetic information in orthogonal chromosomes. XNA polymers are also under active investigation as a source of nuclease resistant affinity reagents (aptamers) and catalysts (xenozymes) with practical applications in disease diagnosis and treatment. In this review, we provide a structural perspective on known antiparallel duplex structures in which at least one strand of the Watson-Crick duplex is composed entirely of XNA. Currently, only a handful of XNA structures have been archived in the Protein Data Bank as compared to the more than 100 000 structures that are now available. Given the growing interest in xenobiology projects, we chose to compare the structural features of XNA polymers and discuss their potential to access new regions of nucleic acid fold space.",
author = "Irina Anosova and Kowal, {Ewa A.} and Dunn, {Matthew R.} and Chaput, {John C.} and {Van Horn}, Wade and Martin Egli",
year = "2015",
doi = "10.1093/nar/gkv1472",
language = "English (US)",
volume = "44",
pages = "1007--1021",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "3",

}

TY - JOUR

T1 - SURVEY AND SUMMARY the structural diversity of artificial genetic polymers

AU - Anosova, Irina

AU - Kowal, Ewa A.

AU - Dunn, Matthew R.

AU - Chaput, John C.

AU - Van Horn, Wade

AU - Egli, Martin

PY - 2015

Y1 - 2015

N2 - Synthetic genetics is a subdiscipline of synthetic biology that aims to develop artificial genetic polymers (also referred to as xeno-nucleic acids or XNAs) that can replicate in vitro and eventually in model cellular organisms. This field of science combines organic chemistry with polymerase engineering to create alternative forms of DNA that can store genetic information and evolve in response to external stimuli. Practitioners of synthetic genetics postulate that XNA could be used to safeguard synthetic biology organisms by storing genetic information in orthogonal chromosomes. XNA polymers are also under active investigation as a source of nuclease resistant affinity reagents (aptamers) and catalysts (xenozymes) with practical applications in disease diagnosis and treatment. In this review, we provide a structural perspective on known antiparallel duplex structures in which at least one strand of the Watson-Crick duplex is composed entirely of XNA. Currently, only a handful of XNA structures have been archived in the Protein Data Bank as compared to the more than 100 000 structures that are now available. Given the growing interest in xenobiology projects, we chose to compare the structural features of XNA polymers and discuss their potential to access new regions of nucleic acid fold space.

AB - Synthetic genetics is a subdiscipline of synthetic biology that aims to develop artificial genetic polymers (also referred to as xeno-nucleic acids or XNAs) that can replicate in vitro and eventually in model cellular organisms. This field of science combines organic chemistry with polymerase engineering to create alternative forms of DNA that can store genetic information and evolve in response to external stimuli. Practitioners of synthetic genetics postulate that XNA could be used to safeguard synthetic biology organisms by storing genetic information in orthogonal chromosomes. XNA polymers are also under active investigation as a source of nuclease resistant affinity reagents (aptamers) and catalysts (xenozymes) with practical applications in disease diagnosis and treatment. In this review, we provide a structural perspective on known antiparallel duplex structures in which at least one strand of the Watson-Crick duplex is composed entirely of XNA. Currently, only a handful of XNA structures have been archived in the Protein Data Bank as compared to the more than 100 000 structures that are now available. Given the growing interest in xenobiology projects, we chose to compare the structural features of XNA polymers and discuss their potential to access new regions of nucleic acid fold space.

UR - http://www.scopus.com/inward/record.url?scp=84964801157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964801157&partnerID=8YFLogxK

U2 - 10.1093/nar/gkv1472

DO - 10.1093/nar/gkv1472

M3 - Article

C2 - 26673703

AN - SCOPUS:84964801157

VL - 44

SP - 1007

EP - 1021

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 3

ER -