Survey and summary

Molecular structures guide the engineering of chromatin

Stefan J. Tekel, Karmella Haynes

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

Chromatin is a system of proteins, RNA, and DNA that interact with each other to organize and regulate genetic information within eukaryotic nuclei. Chromatin proteins carry out essential functions: packing DNA during cell division, partitioning DNA into sub-regions within the nucleus, and controlling levels of gene expression. There is a growing interest in manipulating chromatin dynamics for applications in medicine and agriculture. Progress in this area requires the identification of design rules for the chromatin system. Here, we focus on the relationship between the physical structure and function of chromatin proteins. We discuss key research that has elucidated the intrinsic properties of chromatin proteins and how this information informs design rules for synthetic systems. Recent work demonstrates that chromatin-derived peptide motifs are portable and in some cases can be customized to alter their function. Finally, we present a workflow for fusion protein design and discuss best practices for engineering chromatin to assist scientists in advancing the field of synthetic epigenetics.

Original languageEnglish (US)
Pages (from-to)7555-7570
Number of pages16
JournalNucleic Acids Research
Volume45
Issue number13
DOIs
StatePublished - Jul 1 2017

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Molecular Structure
Chromatin
Proteins
DNA
Workflow
Surveys and Questionnaires
Agriculture
Practice Guidelines
Epigenomics
Cell Division
Medicine
RNA
Gene Expression
Peptides
Research

ASJC Scopus subject areas

  • Genetics

Cite this

Survey and summary : Molecular structures guide the engineering of chromatin. / Tekel, Stefan J.; Haynes, Karmella.

In: Nucleic Acids Research, Vol. 45, No. 13, 01.07.2017, p. 7555-7570.

Research output: Contribution to journalReview article

Tekel, Stefan J. ; Haynes, Karmella. / Survey and summary : Molecular structures guide the engineering of chromatin. In: Nucleic Acids Research. 2017 ; Vol. 45, No. 13. pp. 7555-7570.
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