Surface potential of charged liposomes determined by second harmonic generation

Yan Liu; Elsa C.Y. Yan; Xiaolin Zhao; Kenneth B. Eisenthal

doi:10.1021/la0011634

Surface potential of charged liposomes determined by second harmonic generation

Yan Liu, Elsa C.Y. Yan, Xiaolin Zhao, Kenneth B. Eisenthal

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75 Scopus citations

Abstract

We demonstrate that the surface potential of charged liposomes can be determined by second harmonic generation. The Gouy-Chapman model is used to describe the electric double layer. From the experimental data, we obtained the surface charge density and the surface potential of liposomes consisting of the negatively charged phospholipid dioleoylphosphatidylglycerol. The surface potential was found to be in the range of 20-100 mV depending on the concentration and valence of the electrolyte (NaCl or MgSO₄) in solution. The charge density was found to be ∼1.3 × 10¹⁴ per cm², corresponding to ∼70 Å²/charge, which is comparable to the area per phospholipid headgroup.

Original language	English (US)
Pages (from-to)	2063-2066
Number of pages	4
Journal	Langmuir
Volume	17
Issue number	7
DOIs	https://doi.org/10.1021/la0011634
State	Published - Apr 3 2001
Externally published	Yes

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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10.1021/la0011634

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title = "Surface potential of charged liposomes determined by second harmonic generation",

abstract = "We demonstrate that the surface potential of charged liposomes can be determined by second harmonic generation. The Gouy-Chapman model is used to describe the electric double layer. From the experimental data, we obtained the surface charge density and the surface potential of liposomes consisting of the negatively charged phospholipid dioleoylphosphatidylglycerol. The surface potential was found to be in the range of 20-100 mV depending on the concentration and valence of the electrolyte (NaCl or MgSO4) in solution. The charge density was found to be ∼1.3 × 1014 per cm2, corresponding to ∼70 {\AA}2/charge, which is comparable to the area per phospholipid headgroup.",

author = "Yan Liu and Yan, {Elsa C.Y.} and Xiaolin Zhao and Eisenthal, {Kenneth B.}",

year = "2001",

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doi = "10.1021/la0011634",

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T1 - Surface potential of charged liposomes determined by second harmonic generation

AU - Liu, Yan

AU - Yan, Elsa C.Y.

AU - Zhao, Xiaolin

AU - Eisenthal, Kenneth B.

PY - 2001/4/3

Y1 - 2001/4/3

N2 - We demonstrate that the surface potential of charged liposomes can be determined by second harmonic generation. The Gouy-Chapman model is used to describe the electric double layer. From the experimental data, we obtained the surface charge density and the surface potential of liposomes consisting of the negatively charged phospholipid dioleoylphosphatidylglycerol. The surface potential was found to be in the range of 20-100 mV depending on the concentration and valence of the electrolyte (NaCl or MgSO4) in solution. The charge density was found to be ∼1.3 × 1014 per cm2, corresponding to ∼70 Å2/charge, which is comparable to the area per phospholipid headgroup.

AB - We demonstrate that the surface potential of charged liposomes can be determined by second harmonic generation. The Gouy-Chapman model is used to describe the electric double layer. From the experimental data, we obtained the surface charge density and the surface potential of liposomes consisting of the negatively charged phospholipid dioleoylphosphatidylglycerol. The surface potential was found to be in the range of 20-100 mV depending on the concentration and valence of the electrolyte (NaCl or MgSO4) in solution. The charge density was found to be ∼1.3 × 1014 per cm2, corresponding to ∼70 Å2/charge, which is comparable to the area per phospholipid headgroup.

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Surface potential of charged liposomes determined by second harmonic generation

Abstract

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