Surface potential of charged liposomes determined by second harmonic generation

Yan Liu, Elsa C Y Yan, Xiaolin Zhao, Kenneth B. Eisenthal

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

We demonstrate that the surface potential of charged liposomes can be determined by second harmonic generation. The Gouy-Chapman model is used to describe the electric double layer. From the experimental data, we obtained the surface charge density and the surface potential of liposomes consisting of the negatively charged phospholipid dioleoylphosphatidylglycerol. The surface potential was found to be in the range of 20-100 mV depending on the concentration and valence of the electrolyte (NaCl or MgSO4) in solution. The charge density was found to be ∼1.3 × 1014 per cm2, corresponding to ∼70 Å2/charge, which is comparable to the area per phospholipid headgroup.

Original languageEnglish (US)
Pages (from-to)2063-2066
Number of pages4
JournalLangmuir
Volume17
Issue number7
DOIs
StatePublished - Apr 3 2001
Externally publishedYes

Fingerprint

Liposomes
Surface potential
Harmonic generation
Charge density
Phospholipids
harmonic generations
Surface charge
Electrolytes
electrolytes
valence

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Surface potential of charged liposomes determined by second harmonic generation. / Liu, Yan; Yan, Elsa C Y; Zhao, Xiaolin; Eisenthal, Kenneth B.

In: Langmuir, Vol. 17, No. 7, 03.04.2001, p. 2063-2066.

Research output: Contribution to journalArticle

Liu, Yan ; Yan, Elsa C Y ; Zhao, Xiaolin ; Eisenthal, Kenneth B. / Surface potential of charged liposomes determined by second harmonic generation. In: Langmuir. 2001 ; Vol. 17, No. 7. pp. 2063-2066.
@article{9c4baabd5e0e4ca9b7a9d4926cc38db6,
title = "Surface potential of charged liposomes determined by second harmonic generation",
abstract = "We demonstrate that the surface potential of charged liposomes can be determined by second harmonic generation. The Gouy-Chapman model is used to describe the electric double layer. From the experimental data, we obtained the surface charge density and the surface potential of liposomes consisting of the negatively charged phospholipid dioleoylphosphatidylglycerol. The surface potential was found to be in the range of 20-100 mV depending on the concentration and valence of the electrolyte (NaCl or MgSO4) in solution. The charge density was found to be ∼1.3 × 1014 per cm2, corresponding to ∼70 {\AA}2/charge, which is comparable to the area per phospholipid headgroup.",
author = "Yan Liu and Yan, {Elsa C Y} and Xiaolin Zhao and Eisenthal, {Kenneth B.}",
year = "2001",
month = "4",
day = "3",
doi = "10.1021/la0011634",
language = "English (US)",
volume = "17",
pages = "2063--2066",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "7",

}

TY - JOUR

T1 - Surface potential of charged liposomes determined by second harmonic generation

AU - Liu, Yan

AU - Yan, Elsa C Y

AU - Zhao, Xiaolin

AU - Eisenthal, Kenneth B.

PY - 2001/4/3

Y1 - 2001/4/3

N2 - We demonstrate that the surface potential of charged liposomes can be determined by second harmonic generation. The Gouy-Chapman model is used to describe the electric double layer. From the experimental data, we obtained the surface charge density and the surface potential of liposomes consisting of the negatively charged phospholipid dioleoylphosphatidylglycerol. The surface potential was found to be in the range of 20-100 mV depending on the concentration and valence of the electrolyte (NaCl or MgSO4) in solution. The charge density was found to be ∼1.3 × 1014 per cm2, corresponding to ∼70 Å2/charge, which is comparable to the area per phospholipid headgroup.

AB - We demonstrate that the surface potential of charged liposomes can be determined by second harmonic generation. The Gouy-Chapman model is used to describe the electric double layer. From the experimental data, we obtained the surface charge density and the surface potential of liposomes consisting of the negatively charged phospholipid dioleoylphosphatidylglycerol. The surface potential was found to be in the range of 20-100 mV depending on the concentration and valence of the electrolyte (NaCl or MgSO4) in solution. The charge density was found to be ∼1.3 × 1014 per cm2, corresponding to ∼70 Å2/charge, which is comparable to the area per phospholipid headgroup.

UR - http://www.scopus.com/inward/record.url?scp=0035799433&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035799433&partnerID=8YFLogxK

U2 - 10.1021/la0011634

DO - 10.1021/la0011634

M3 - Article

AN - SCOPUS:0035799433

VL - 17

SP - 2063

EP - 2066

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 7

ER -