Abstract

We report a new surface plasmon resonance (SPR) protein sensor using the Vroman effect for real-time, sensitive and selective detection of protein. The sensor relies on the competitive nature of protein adsorption onto the surface, directly depending upon protein's molecular weight. The sensor uses SPR for highly sensitive biomolecular interactions detection and the Vroman effect for highly selective detection. By using the Vroman effect we bypass having to rely on bio-receptors and their attachment to transducers, a process known to be complex and time-consuming. The protein sensor is microfabricated to perform real-time protein detection using four different proteins including aprotinin (0.65 kDa), lysozyme (14.7 kDa), streptavidine (53 kDa), and isolectin (114 kDa) on three different surfaces, namely a bare-gold surface and two others modified by OH- and COOH-terminated self-assembled monolayer (SAM). The real-time adsorption and displacement of the proteins are observed by SPR and evaluated using an atomic force microscope (AFM). The sensor can distinguish proteins of at least 14.05 kDa in molecular weight and demonstrate a very low false positive rate. The protein detector can be integrated with microfluidic systems to provide extremely sensitive and selective analytical capability.

Original languageEnglish (US)
Pages (from-to)893-899
Number of pages7
JournalBiosensors and Bioelectronics
Volume24
Issue number4
DOIs
StatePublished - Dec 1 2008

Fingerprint

Surface Plasmon Resonance
Surface plasmon resonance
Proteins
Sensors
Adsorption
Molecular weight
Molecular Weight
Aprotinin
Self assembled monolayers
Microfluidics
Muramidase
Lectins
Gold
Transducers
Microscopes
Enzymes
Detectors

Keywords

  • Atomic force microscope (AFM)
  • Microfluidics
  • Protein sensor
  • Self-assembled monolayer (SAM)
  • Surface plasmon resonance (SPR)
  • Vroman effect

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Surface plasmon resonance protein sensor using Vroman effect. / Choi, Seokheun; Yang, Yongmo; Chae, Junseok.

In: Biosensors and Bioelectronics, Vol. 24, No. 4, 01.12.2008, p. 893-899.

Research output: Contribution to journalArticle

Choi, Seokheun ; Yang, Yongmo ; Chae, Junseok. / Surface plasmon resonance protein sensor using Vroman effect. In: Biosensors and Bioelectronics. 2008 ; Vol. 24, No. 4. pp. 893-899.
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