Surface plasmon resonance mass spectrometry for protein analysis.

Dobrin Nedelkov; Randall W. Nelson

doi:10.1385/1-59745-026-x:131

Surface plasmon resonance mass spectrometry for protein analysis.

Dobrin Nedelkov, Randall W. Nelson

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The combination of surface plasmon pesonance (SPR) and mass spectrometry (MS) provides a unique methodology for studying proteins and their interactions. SPR is utilized to assess protein quantitative variations and the kinetic aspects of protein interactions, whereas MS complements the analysis by providing an exclusive look at the structural features of the interacting proteins via measurement of their mass. Thus, intrinsic protein structural modifications that go unregistered via the SPR detection can readily be assessed from the MS data. The purpose of this chapter is dissemination of the procedures and protocols for successful SPR-MS analysis. The individual steps of the complete SPRMS process are illustrated via analysis of cardiac troponin I (cTnI).

Original language	English (US)
Pages (from-to)	131-139
Number of pages	9
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	328
DOIs	https://doi.org/10.1385/1-59745-026-x:131
State	Published - 2006

ASJC Scopus subject areas

Molecular Biology
Genetics

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abstract = "The combination of surface plasmon pesonance (SPR) and mass spectrometry (MS) provides a unique methodology for studying proteins and their interactions. SPR is utilized to assess protein quantitative variations and the kinetic aspects of protein interactions, whereas MS complements the analysis by providing an exclusive look at the structural features of the interacting proteins via measurement of their mass. Thus, intrinsic protein structural modifications that go unregistered via the SPR detection can readily be assessed from the MS data. The purpose of this chapter is dissemination of the procedures and protocols for successful SPR-MS analysis. The individual steps of the complete SPRMS process are illustrated via analysis of cardiac troponin I (cTnI).",

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Surface plasmon resonance mass spectrometry for protein analysis.

Abstract

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