Surface Plasmon Resonance Biomolecular Interaction Analysis Mass Spectrometry. 2. Fiber Optic-Based Analysis

Randall W. Nelson, Jennifer R. Krone, Osten Jansson

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Fiber optic probe-based surface plasmon resonance (SPR) detection has been used in combination with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry for the rapid, sensitive, and selective detection of biomolecules. SPR was used to monitor the covalent immobilization of a polyclonal anti-body to the surface of a fiber optic probe. The derivatized probe was then used for the selective detection (from solution) of the corresponding antigen and a secondary antibody directed toward the antigen. Species retained during the SPR detection process were next analyzed by direct MALDI-TOF analysis of the probe surface (after exposed to the MALDI matrix and introduction into the mass spectrometer). The combined approach allowed for die two-dimensional detection of biomolecules, with SPR analysis yielding quantitative information pertinent to the binding events and MALDI-TOF providing details on the qualitative nature of the binding partners.

Original languageEnglish (US)
Pages (from-to)4369-4374
Number of pages6
JournalAnalytical chemistry
Volume69
Issue number21
DOIs
StatePublished - Nov 1 1997

ASJC Scopus subject areas

  • Analytical Chemistry

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