Surface Plasmon Resonance Biomolecular Interaction Analysis Mass Spectrometry. 1. Chip-Based Analysis

Randall W. Nelson, Jennifer R. Krone, Osten Jansson

Research output: Contribution to journalArticle

122 Scopus citations

Abstract

The use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) in concert with surface plasmon resonance-based biomolecular interaction analysis (SPR-BIA) is reported. A chip-based biosensor unit was used to simultaneously monitor biomolecular interactions taking place on four different regions of the sensor chip (flow cells). Species retained during SPR-BIA were then identified by performing MALDI-TOF directly from within the area of the flow cells. Analyses were performed on an antibody/antigen/anti-body system with detection limits in the low-femtomole range. The combined assay demonstrates the use of SPR-BIA to evaluate the relative stability of sequential solution-phase interactions, as well as, upon MALDI-TOF analysis, the ability to unambiguously confirm the presence of species retained during the interaction analysis.

Original languageEnglish (US)
Pages (from-to)4363-4368
Number of pages6
JournalAnalytical chemistry
Volume69
Issue number21
DOIs
StatePublished - Nov 1 1997

ASJC Scopus subject areas

  • Analytical Chemistry

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