Surface modification of graphene nanopores for protein translocation

Y. P. Shan, P. B. Tiwari, P. Krishnakumar, I. Vlassiouk, W. Z. Li, X. W. Wang, Y. Darici, Stuart Lindsay, H. D. Wang, S. Smirnov, J. He

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Studies of DNA translocation through graphene nanopores have revealed their potential for DNA sequencing. Here we report a study of protein translocation through chemically modified graphene nanopores. A transmission electron microscope (TEM) was used to cut nanopores with diameters between 5 and 20 nm in multilayer graphene prepared by chemical vapor deposition (CVD). After oxygen plasma treatment, the dependence of the measured ionic current on salt concentration and pH was consistent with a small surface charge induced by the formation of carboxyl groups. While translocation of gold nanoparticles (10 nm) was readily detected through such treated pores of a larger diameter, translocation of the protein ferritin was not observed either for oxygen plasma treated pores, or for pores modified with mercaptohexadecanoic acid. Ferritin translocation events were reliably observed after the pores were modified with the phospholipid-PEG (DPPE-PEG750) amphiphile. The ion current signature of translocation events was complex, suggesting that a series of interactions between the protein and pores occurs during the process.

Original languageEnglish (US)
Article number495102
JournalNanotechnology
Volume24
Issue number49
DOIs
StatePublished - Dec 13 2013

Fingerprint

Nanopores
Graphite
Graphene
Surface treatment
Ferritins
Proteins
DNA
Oxygen
Plasmas
Porins
Amphiphiles
Phospholipids
Surface charge
Gold
Polyethylene glycols
Chemical vapor deposition
Multilayers
Electron microscopes
Salts
Ions

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

Shan, Y. P., Tiwari, P. B., Krishnakumar, P., Vlassiouk, I., Li, W. Z., Wang, X. W., ... He, J. (2013). Surface modification of graphene nanopores for protein translocation. Nanotechnology, 24(49), [495102]. https://doi.org/10.1088/0957-4484/24/49/495102

Surface modification of graphene nanopores for protein translocation. / Shan, Y. P.; Tiwari, P. B.; Krishnakumar, P.; Vlassiouk, I.; Li, W. Z.; Wang, X. W.; Darici, Y.; Lindsay, Stuart; Wang, H. D.; Smirnov, S.; He, J.

In: Nanotechnology, Vol. 24, No. 49, 495102, 13.12.2013.

Research output: Contribution to journalArticle

Shan, YP, Tiwari, PB, Krishnakumar, P, Vlassiouk, I, Li, WZ, Wang, XW, Darici, Y, Lindsay, S, Wang, HD, Smirnov, S & He, J 2013, 'Surface modification of graphene nanopores for protein translocation', Nanotechnology, vol. 24, no. 49, 495102. https://doi.org/10.1088/0957-4484/24/49/495102
Shan YP, Tiwari PB, Krishnakumar P, Vlassiouk I, Li WZ, Wang XW et al. Surface modification of graphene nanopores for protein translocation. Nanotechnology. 2013 Dec 13;24(49). 495102. https://doi.org/10.1088/0957-4484/24/49/495102
Shan, Y. P. ; Tiwari, P. B. ; Krishnakumar, P. ; Vlassiouk, I. ; Li, W. Z. ; Wang, X. W. ; Darici, Y. ; Lindsay, Stuart ; Wang, H. D. ; Smirnov, S. ; He, J. / Surface modification of graphene nanopores for protein translocation. In: Nanotechnology. 2013 ; Vol. 24, No. 49.
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