Suppressor and novel mutants of bacteriophage T4 tRNAGly

William H. McClain, K. Foss, Jay Schneider, Cecilia Guerrier-Takada, Sidney Altman

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We have isolated a weak UGA suppressor of phage T4 tRNAGly in which the anticodon is changed from UCC to UCA. Two secondary mutants lacking suppressor activity are atypical in accumulating tRNAGly. Both mutations change the T stem of the cloverleaf model. One involves a G to A change at the 5′ base position of the middle base-pair; the second involves a C to U change at a constant base position next to the T loop. The precursor RNAs of the mutants were cleaved in vitro with the catalytic RNA subunit of RNase P. Relative to normal precursor RNA, the precursor mutated at the middle base-pair position of the T stem was cleaved more rapidly, whereas the precursor mutated at the base-pair position next to the T loop was cleaved more slowly.

Original languageEnglish (US)
Pages (from-to)223-226
Number of pages4
JournalJournal of molecular biology
Volume193
Issue number1
DOIs
StatePublished - Jan 5 1987
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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