TY - JOUR
T1 - Suppressor and novel mutants of bacteriophage T4 tRNAGly
AU - McClain, William H.
AU - Foss, K.
AU - Schneider, Jay
AU - Guerrier-Takada, Cecilia
AU - Altman, Sidney
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1987/1/5
Y1 - 1987/1/5
N2 - We have isolated a weak UGA suppressor of phage T4 tRNAGly in which the anticodon is changed from UCC to UCA. Two secondary mutants lacking suppressor activity are atypical in accumulating tRNAGly. Both mutations change the T stem of the cloverleaf model. One involves a G to A change at the 5′ base position of the middle base-pair; the second involves a C to U change at a constant base position next to the T loop. The precursor RNAs of the mutants were cleaved in vitro with the catalytic RNA subunit of RNase P. Relative to normal precursor RNA, the precursor mutated at the middle base-pair position of the T stem was cleaved more rapidly, whereas the precursor mutated at the base-pair position next to the T loop was cleaved more slowly.
AB - We have isolated a weak UGA suppressor of phage T4 tRNAGly in which the anticodon is changed from UCC to UCA. Two secondary mutants lacking suppressor activity are atypical in accumulating tRNAGly. Both mutations change the T stem of the cloverleaf model. One involves a G to A change at the 5′ base position of the middle base-pair; the second involves a C to U change at a constant base position next to the T loop. The precursor RNAs of the mutants were cleaved in vitro with the catalytic RNA subunit of RNase P. Relative to normal precursor RNA, the precursor mutated at the middle base-pair position of the T stem was cleaved more rapidly, whereas the precursor mutated at the base-pair position next to the T loop was cleaved more slowly.
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U2 - 10.1016/0022-2836(87)90640-1
DO - 10.1016/0022-2836(87)90640-1
M3 - Article
C2 - 2438421
AN - SCOPUS:0023644304
SN - 0022-2836
VL - 193
SP - 223
EP - 226
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 1
ER -