TY - JOUR
T1 - Suppression of water-bloom cyanobacterium Microcystis aeruginosa by algaecide hydrogen peroxide maximized through programmed cell death
AU - Zhou, Tingru
AU - Cao, Huansheng
AU - Zheng, Jie
AU - Teng, Fei
AU - Wang, Xuejian
AU - Lou, Kai
AU - Zhang, Xihui
AU - Tao, Yi
N1 - Funding Information:
Funding: National Natural Science Foundation of China ( 51108244 ), Major Science and Technology Program for Water Pollution Control and Treatment ( 2017ZX07202 ), the National Key Research and Development Program of China ( 2018YFC0406304 ), the Development and Reform Commission of Shenzhen Municipality (urban water recycling and environment safety program), and Tsinghua University (School of Environment) , Xingrong Environment Holding Ltd. Joint Research Center for Advanced Water Technology are greatly acknowledged.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/7/5
Y1 - 2020/7/5
N2 - The global expansion and intensification of toxic cyanobacterial blooms require effective algaecides. Algaecides should be selective, effective, fast-acting, and ideally suppress cyanotoxin production. In this study, whether both maximum growth suppression and minimal toxin production can be simultaneously achieved was tested with a selective algaecide H2O2, through its ability to induce apoptosis-like programmed cell death (AL PCD) in a common bloom species Microcystis aeruginosa. Under doses of 1−15 mg L−1, non-monotonic dose-response suppression of H2O2 on M. aeruginosa were observed, where maximal cell death and minimal microcystin production both occurred at a moderate dose of 10 mg L−1 H2O2. Maximal cell death was indeed achieved through AL PCD, as revealed by integrated biochemical, structural, physiological and transcriptional evidence; transcriptional profile suggested AL PCD was mediated by mazEF and lexA systems. Higher H2O2 doses directly led to necrosis in M. aeruginosa, while lower doses only caused recoverable stress. The integrated data showed the choice between the two modes of cell death is determined by the intracellular energy state under stress. A model was proposed for suppressing M. aeruginosa with AL PCD or necrosis. H2O2 was demonstrated to simultaneously maximize the suppression of both growth and microcystin production through triggering AL PCD.
AB - The global expansion and intensification of toxic cyanobacterial blooms require effective algaecides. Algaecides should be selective, effective, fast-acting, and ideally suppress cyanotoxin production. In this study, whether both maximum growth suppression and minimal toxin production can be simultaneously achieved was tested with a selective algaecide H2O2, through its ability to induce apoptosis-like programmed cell death (AL PCD) in a common bloom species Microcystis aeruginosa. Under doses of 1−15 mg L−1, non-monotonic dose-response suppression of H2O2 on M. aeruginosa were observed, where maximal cell death and minimal microcystin production both occurred at a moderate dose of 10 mg L−1 H2O2. Maximal cell death was indeed achieved through AL PCD, as revealed by integrated biochemical, structural, physiological and transcriptional evidence; transcriptional profile suggested AL PCD was mediated by mazEF and lexA systems. Higher H2O2 doses directly led to necrosis in M. aeruginosa, while lower doses only caused recoverable stress. The integrated data showed the choice between the two modes of cell death is determined by the intracellular energy state under stress. A model was proposed for suppressing M. aeruginosa with AL PCD or necrosis. H2O2 was demonstrated to simultaneously maximize the suppression of both growth and microcystin production through triggering AL PCD.
KW - Apoptosis-like programmed cell death
KW - Hydrogen peroxide
KW - Microcystis
KW - Non-monotonic dose-response
KW - Transcriptome
KW - microcysin-LR
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U2 - 10.1016/j.jhazmat.2020.122394
DO - 10.1016/j.jhazmat.2020.122394
M3 - Article
C2 - 32114135
AN - SCOPUS:85080049256
SN - 0304-3894
VL - 393
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 122394
ER -