1H NMR study of the effects of sample contamination in the metabolomic analysis of mouse urine

Haiwei Gu; Zhengzheng Pan; Chester Duda; Doug Mann; Candice Kissinger; Candace Rohde; Daniel Raftery

doi:10.1016/j.jpba.2007.06.030

¹H NMR study of the effects of sample contamination in the metabolomic analysis of mouse urine

Haiwei Gu, Zhengzheng Pan, Chester Duda, Doug Mann, Candice Kissinger, Candace Rohde, Daniel Raftery

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Nuclear magnetic resonance (NMR) spectroscopy was used to evaluate and optimize the strategy for collecting mouse urine samples. A series of normal urine samples and those mixed with folate-deficient food, turkey or mouse fecal particles were analyzed using principal component analysis (PCA). The metabolic profile of urine mixed with folate-deficient food was found to be extremely different than that of clean urine. Changes in the urine composition caused by mixing with turkey or feces are relatively small as judged by the output of PCA. As a result, turkey may be considered as an applicable food source for obtaining uncontaminated urine samples for metabolomics-based research.

Original language	English (US)
Pages (from-to)	134-140
Number of pages	7
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	45
Issue number	1
DOIs	https://doi.org/10.1016/j.jpba.2007.06.030
State	Published - Sep 21 2007
Externally published	Yes

Keywords

Diet
Metabolomics
Mouse urine
Nuclear magnetic resonance (NMR)
Principal component analysis (PCA)

ASJC Scopus subject areas

Analytical Chemistry
Pharmaceutical Science
Drug Discovery
Spectroscopy
Clinical Biochemistry

Access to Document

10.1016/j.jpba.2007.06.030

Cite this

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title = "1H NMR study of the effects of sample contamination in the metabolomic analysis of mouse urine",

abstract = "Nuclear magnetic resonance (NMR) spectroscopy was used to evaluate and optimize the strategy for collecting mouse urine samples. A series of normal urine samples and those mixed with folate-deficient food, turkey or mouse fecal particles were analyzed using principal component analysis (PCA). The metabolic profile of urine mixed with folate-deficient food was found to be extremely different than that of clean urine. Changes in the urine composition caused by mixing with turkey or feces are relatively small as judged by the output of PCA. As a result, turkey may be considered as an applicable food source for obtaining uncontaminated urine samples for metabolomics-based research.",

keywords = "Diet, Metabolomics, Mouse urine, Nuclear magnetic resonance (NMR), Principal component analysis (PCA)",

author = "Haiwei Gu and Zhengzheng Pan and Chester Duda and Doug Mann and Candice Kissinger and Candace Rohde and Daniel Raftery",

note = "Funding Information: Financial support : This work was supported by the NIH Roadmap Initiative on Metabolomics Technology, NIH/NIDDK 3 R21 DK070290-01. ",

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AU - Gu, Haiwei

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AU - Duda, Chester

AU - Mann, Doug

AU - Kissinger, Candice

AU - Rohde, Candace

AU - Raftery, Daniel

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AB - Nuclear magnetic resonance (NMR) spectroscopy was used to evaluate and optimize the strategy for collecting mouse urine samples. A series of normal urine samples and those mixed with folate-deficient food, turkey or mouse fecal particles were analyzed using principal component analysis (PCA). The metabolic profile of urine mixed with folate-deficient food was found to be extremely different than that of clean urine. Changes in the urine composition caused by mixing with turkey or feces are relatively small as judged by the output of PCA. As a result, turkey may be considered as an applicable food source for obtaining uncontaminated urine samples for metabolomics-based research.

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