Succinate

quinol oxidoreductases in the cyanobacterium Synechocystis sp. strain PCC 6803: Presence and function in metabolism and electron transport

Jason W. Cooley, Crispin A. Howitt, Willem Vermaas

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The open reading frames sll1625 and sll0823, which have significant sequence similarity to genes coding for the FeS subunits of succinate dehydrogenase and fumarate reductase, were deleted singly and in combination in the cyanobacterium Synechocystis sp. strain PCC 6803. When the organic acid content in the Δsll1625 and Δsll0823 strains was analyzed, a 100-fold decrease in succinate and fumarate concentrations was observed relative to the wild type. A similar analysis for the Δsll1625 Δsll0823 strain revealed that 17% of the wild-type succinate levels remained, while only 1 to 2% of the wild-type fumarate levels were present. Addition of 2-oxoglutarate to the growth media of the double mutant strain prior to analysis of organic acids in cells caused succinate to accumulate. This indicates that succinate dehydrogenase activity had been blocked by the deletions and that 2- oxoglutarate can be converted to succinate in vivo in this organism, even though a traditional 2-oxoglutarate dehydrogenase is lacking. In addition, reduction of the thylakoid plastoquinone pool in darkness in the presence of KCN was up to fivefold slower in the mutants than in the wild type. Moreover, in vitro succinate dehydrogenase activity observed in wild-type membranes is absent from those isolated from the double mutant and reduced in those from the single mutants, further indicating that the sll1625 and sll0823 open reading frames encode subunits of succinate dehydrogenase complexes that are active in the thylakoid membrane of the cyanobacterium.

Original languageEnglish (US)
Pages (from-to)714-722
Number of pages9
JournalJournal of Bacteriology
Volume182
Issue number3
DOIs
StatePublished - Feb 2000

Fingerprint

Hydroquinones
Synechocystis
Succinate Dehydrogenase
Cyanobacteria
Succinic Acid
Electron Transport
Oxidoreductases
Fumarates
Thylakoids
Open Reading Frames
Plastoquinone
Ketoglutarate Dehydrogenase Complex
Acids
Darkness
Membranes
Growth
Genes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Succinate : quinol oxidoreductases in the cyanobacterium Synechocystis sp. strain PCC 6803: Presence and function in metabolism and electron transport. / Cooley, Jason W.; Howitt, Crispin A.; Vermaas, Willem.

In: Journal of Bacteriology, Vol. 182, No. 3, 02.2000, p. 714-722.

Research output: Contribution to journalArticle

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abstract = "The open reading frames sll1625 and sll0823, which have significant sequence similarity to genes coding for the FeS subunits of succinate dehydrogenase and fumarate reductase, were deleted singly and in combination in the cyanobacterium Synechocystis sp. strain PCC 6803. When the organic acid content in the Δsll1625 and Δsll0823 strains was analyzed, a 100-fold decrease in succinate and fumarate concentrations was observed relative to the wild type. A similar analysis for the Δsll1625 Δsll0823 strain revealed that 17{\%} of the wild-type succinate levels remained, while only 1 to 2{\%} of the wild-type fumarate levels were present. Addition of 2-oxoglutarate to the growth media of the double mutant strain prior to analysis of organic acids in cells caused succinate to accumulate. This indicates that succinate dehydrogenase activity had been blocked by the deletions and that 2- oxoglutarate can be converted to succinate in vivo in this organism, even though a traditional 2-oxoglutarate dehydrogenase is lacking. In addition, reduction of the thylakoid plastoquinone pool in darkness in the presence of KCN was up to fivefold slower in the mutants than in the wild type. Moreover, in vitro succinate dehydrogenase activity observed in wild-type membranes is absent from those isolated from the double mutant and reduced in those from the single mutants, further indicating that the sll1625 and sll0823 open reading frames encode subunits of succinate dehydrogenase complexes that are active in the thylakoid membrane of the cyanobacterium.",
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