Subventricular zone neural progenitors from rapid brain autopsies of elderly subjects with and without neurodegenerative disease

Brian W. Leonard, Diego Mastroeni, Andrew Grover, Qiang Liu, Kechun Yang, Ming Gao, Jie Wu, David Pootrakul, Simone A. van den Berge, Elly M. Hol, Joseph Rogers

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In mice and in young adult humans, the subventricular zone (SVZ) contains multipotent, dividing astrocytes, some of which, when cultured, produce neurospheres that differentiate into neurons and glia. It is unknown whether the SVZ of very old humans has this capacity. Here, we report that neural stem/progenitor cells can also be cultured from rapid autopsy samples of SVZ from elderly human subjects, including patients with age-related neurologic disorders. Histological sections of SVZ from these cases showed a glial fibrillary acidic protein (GFAP)-positive ribbon of astrocytes similar to the astrocyte ribbon in human periventricular white matter biopsies that is reported to be a rich source of neural progenitors. Cultures of the SVZ contained 1) neurospheres with a core of Musashi-1-, nestin-, and nucleostemin-immunopositive cells as well as more differentiated GFAP-positive astrocytes; 2) SMI-311-, MAP2a/b-, and β-tubulin(III)-positive neurons; and 3) galactocerebroside-positive oligodendrocytes. Neurospheres continued to generate differentiated progeny for months after primary culturing, in some cases nearly 2 years postinitial plating. Patch clamp studies of differentiated SVZ cells expressing neuron-specific antigens revealed voltage-dependent, tetrodotoxin-sensitive, inward Na+ currents and voltage-dependent, delayed, slowly inactivating K+ currents, electrophysiologic characteristics of neurons. A subpopulation of these cells also exhibited responses consistent with the kinetics and pharmacology of the h-current. However, although these cells displayed some aspects of neuronal function, they remained immature, insofar as they did not fire action potentials. These studies suggest that human neural progenitor activity may remain viable throughout much of the life span, even in the face of severe neurodegenerative disease.

Original languageEnglish (US)
Pages (from-to)269-294
Number of pages26
JournalJournal of Comparative Neurology
Volume515
Issue number3
DOIs
StatePublished - Jul 20 2009
Externally publishedYes

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Lateral Ventricles
Neurodegenerative Diseases
Autopsy
Astrocytes
Brain
Neurons
Glial Fibrillary Acidic Protein
Nestin
Neural Stem Cells
Oligodendroglia
Tetrodotoxin
Tubulin
Nervous System Diseases
Neuroglia
Action Potentials
Young Adult
Stem Cells
Pharmacology
Biopsy
Antigens

Keywords

  • Alzheimer's disease
  • Neural precursors
  • Neural stem cells
  • Neuronal differentiation
  • Neurospheres

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Subventricular zone neural progenitors from rapid brain autopsies of elderly subjects with and without neurodegenerative disease. / Leonard, Brian W.; Mastroeni, Diego; Grover, Andrew; Liu, Qiang; Yang, Kechun; Gao, Ming; Wu, Jie; Pootrakul, David; van den Berge, Simone A.; Hol, Elly M.; Rogers, Joseph.

In: Journal of Comparative Neurology, Vol. 515, No. 3, 20.07.2009, p. 269-294.

Research output: Contribution to journalArticle

Leonard, BW, Mastroeni, D, Grover, A, Liu, Q, Yang, K, Gao, M, Wu, J, Pootrakul, D, van den Berge, SA, Hol, EM & Rogers, J 2009, 'Subventricular zone neural progenitors from rapid brain autopsies of elderly subjects with and without neurodegenerative disease', Journal of Comparative Neurology, vol. 515, no. 3, pp. 269-294. https://doi.org/10.1002/cne.22040
Leonard, Brian W. ; Mastroeni, Diego ; Grover, Andrew ; Liu, Qiang ; Yang, Kechun ; Gao, Ming ; Wu, Jie ; Pootrakul, David ; van den Berge, Simone A. ; Hol, Elly M. ; Rogers, Joseph. / Subventricular zone neural progenitors from rapid brain autopsies of elderly subjects with and without neurodegenerative disease. In: Journal of Comparative Neurology. 2009 ; Vol. 515, No. 3. pp. 269-294.
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