Subsurface processes influence oxidant availability and chemoautotrophic hydrogen metabolism in Yellowstone hot springs

Melody R. Lindsay, Maximiliano J. Amenabar, Kristopher M. Fecteau, Randal V. Debes, Maria C. Fernandes Martins, Kirsten E. Fristad, Huifang Xu, Tori M. Hoehler, Everett Shock, Eric S. Boyd

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The geochemistry of hot springs and the availability of oxidants capable of supporting microbial metabolisms are influenced by subsurface processes including the separation of hydrothermal fluids into vapor and liquid phases. Here, we characterized the influence of geochemical variation and oxidant availability on the abundance, composition, and activity of hydrogen (H2)-dependent chemoautotrophs along the outflow channels of two-paired hot springs in Yellowstone National Park. The hydrothermal fluid at Roadside East (RSE; 82.4°C, pH 3.0) is acidic due to vapor-phase input while the fluid at Roadside West (RSW; 68.1°C, pH 7.0) is circumneutral due to liquid-phase input. Most chemotrophic communities exhibited net rates of H2 oxidation, consistent with H2 support of primary productivity, with one chemotrophic community exhibiting a net rate of H2 production. Abundant H2-oxidizing chemoautotrophs were supported by reduction in oxygen, elemental sulfur, sulfate, and nitrate in RSW and oxygen and ferric iron in RSE; O2 utilizing hydrogenotrophs increased in abundance down both outflow channels. Sequencing of 16S rRNA transcripts or genes from native sediments and dilution series incubations, respectively, suggests that members of the archaeal orders Sulfolobales, Desulfurococcales, and Thermoproteales are likely responsible for H2 oxidation in RSE, whereas members of the bacterial order Thermoflexales and the archaeal order Thermoproteales are likely responsible for H2 oxidation in RSW. These observations suggest that subsurface processes strongly influence spring chemistry and oxidant availability, which in turn select for unique assemblages of H2 oxidizing microorganisms. Therefore, these data point to the role of oxidant availability in shaping the ecology and evolution of hydrogenotrophic organisms.

Original languageEnglish (US)
JournalGeobiology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

hot springs
thermal spring
oxidants
oxidant
hydrogen
metabolism
oxidation
hydrothermal fluid
vapors
outflow
oxygen
hydrogen production
liquid
liquids
geochemistry
primary productivity
national parks
sulfates
sulfur
chemistry

Keywords

  • Boiling
  • Chemosynthesis
  • Electron acceptor
  • Hydrogen
  • Oxidant
  • Phase separation
  • Primary production

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Environmental Science(all)
  • Earth and Planetary Sciences(all)

Cite this

Lindsay, M. R., Amenabar, M. J., Fecteau, K. M., Debes, R. V., Fernandes Martins, M. C., Fristad, K. E., ... Boyd, E. S. (Accepted/In press). Subsurface processes influence oxidant availability and chemoautotrophic hydrogen metabolism in Yellowstone hot springs. Geobiology. https://doi.org/10.1111/gbi.12308

Subsurface processes influence oxidant availability and chemoautotrophic hydrogen metabolism in Yellowstone hot springs. / Lindsay, Melody R.; Amenabar, Maximiliano J.; Fecteau, Kristopher M.; Debes, Randal V.; Fernandes Martins, Maria C.; Fristad, Kirsten E.; Xu, Huifang; Hoehler, Tori M.; Shock, Everett; Boyd, Eric S.

In: Geobiology, 01.01.2018.

Research output: Contribution to journalArticle

Lindsay, MR, Amenabar, MJ, Fecteau, KM, Debes, RV, Fernandes Martins, MC, Fristad, KE, Xu, H, Hoehler, TM, Shock, E & Boyd, ES 2018, 'Subsurface processes influence oxidant availability and chemoautotrophic hydrogen metabolism in Yellowstone hot springs', Geobiology. https://doi.org/10.1111/gbi.12308
Lindsay, Melody R. ; Amenabar, Maximiliano J. ; Fecteau, Kristopher M. ; Debes, Randal V. ; Fernandes Martins, Maria C. ; Fristad, Kirsten E. ; Xu, Huifang ; Hoehler, Tori M. ; Shock, Everett ; Boyd, Eric S. / Subsurface processes influence oxidant availability and chemoautotrophic hydrogen metabolism in Yellowstone hot springs. In: Geobiology. 2018.
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AB - The geochemistry of hot springs and the availability of oxidants capable of supporting microbial metabolisms are influenced by subsurface processes including the separation of hydrothermal fluids into vapor and liquid phases. Here, we characterized the influence of geochemical variation and oxidant availability on the abundance, composition, and activity of hydrogen (H2)-dependent chemoautotrophs along the outflow channels of two-paired hot springs in Yellowstone National Park. The hydrothermal fluid at Roadside East (RSE; 82.4°C, pH 3.0) is acidic due to vapor-phase input while the fluid at Roadside West (RSW; 68.1°C, pH 7.0) is circumneutral due to liquid-phase input. Most chemotrophic communities exhibited net rates of H2 oxidation, consistent with H2 support of primary productivity, with one chemotrophic community exhibiting a net rate of H2 production. Abundant H2-oxidizing chemoautotrophs were supported by reduction in oxygen, elemental sulfur, sulfate, and nitrate in RSW and oxygen and ferric iron in RSE; O2 utilizing hydrogenotrophs increased in abundance down both outflow channels. Sequencing of 16S rRNA transcripts or genes from native sediments and dilution series incubations, respectively, suggests that members of the archaeal orders Sulfolobales, Desulfurococcales, and Thermoproteales are likely responsible for H2 oxidation in RSE, whereas members of the bacterial order Thermoflexales and the archaeal order Thermoproteales are likely responsible for H2 oxidation in RSW. These observations suggest that subsurface processes strongly influence spring chemistry and oxidant availability, which in turn select for unique assemblages of H2 oxidizing microorganisms. Therefore, these data point to the role of oxidant availability in shaping the ecology and evolution of hydrogenotrophic organisms.

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