Subcellular Compartmentalization and Trafficking of the Biosynthetic Machinery for Fungal Melanin

Srijana Upadhyay, Xinping Xu, David Lowry, Jennifer C. Jackson, Robert Roberson, Xiaorong Lin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Protection by melanin depends on its subcellular location. Although most filamentous fungi synthesize melanin via a polyketide synthase pathway, where and how melanin biosynthesis occurs and how it is deposited as extracellular granules remain elusive. Using a forward genetic screen in the pathogen Aspergillus fumigatus, we find that mutations in an endosomal sorting nexin abolish melanin cell-wall deposition. We find that all enzymes involved in the early steps of melanin biosynthesis are recruited to endosomes through a non-conventional secretory pathway. In contrast, late melanin enzymes accumulate in the cell wall. Such subcellular compartmentalization of the melanin biosynthetic machinery occurs in both A. fumigatus and A. nidulans. Thus, fungal melanin biosynthesis appears to be initiated in endosomes with exocytosis leading to melanin extracellular deposition, much like the synthesis and trafficking of mammalian melanin in endosomally derived melanosomes.

Original languageEnglish (US)
Pages (from-to)2511-2518
Number of pages8
JournalCell Reports
Volume14
Issue number11
DOIs
StatePublished - Mar 22 2016

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Melanins
Machinery
Biosynthesis
Aspergillus fumigatus
Endosomes
Cell Wall
Sorting Nexins
Polyketide Synthases
Melanosomes
Aspergillus
Secretory Pathway
Exocytosis
Pathogens
Enzymes
Fungi
Cells
Mutation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Subcellular Compartmentalization and Trafficking of the Biosynthetic Machinery for Fungal Melanin. / Upadhyay, Srijana; Xu, Xinping; Lowry, David; Jackson, Jennifer C.; Roberson, Robert; Lin, Xiaorong.

In: Cell Reports, Vol. 14, No. 11, 22.03.2016, p. 2511-2518.

Research output: Contribution to journalArticle

Upadhyay, Srijana ; Xu, Xinping ; Lowry, David ; Jackson, Jennifer C. ; Roberson, Robert ; Lin, Xiaorong. / Subcellular Compartmentalization and Trafficking of the Biosynthetic Machinery for Fungal Melanin. In: Cell Reports. 2016 ; Vol. 14, No. 11. pp. 2511-2518.
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