Styrene biosynthesis from glucose by engineered E. Coli

Rebekah McKenna, David Nielsen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Styrene is a large volume, commodity petrochemical with diverse commercial applications, including as a monomer building-block for the synthesis of many useful polymers. Here we demonstrate how, through the de novo design and development of a novel metabolic pathway, styrene can alternatively be synthesized from renewable substrates such as glucose. The conversion of endogenously synthesized L-phenylalanine to styrene was achieved by the co-expression of phenylalanine ammonia lyase and trans-cinnamate decarboxylase. Candidate isoenzymes for each step were screened from bacterial, yeast, and plant genetic sources. Finally, over-expression of PAL2 from Arabidopsis thaliana and FDC1 from Saccharomyces cerevisiae (originally classified as ferulate decarboxylase) in an L-phenylalanine over-producing Escherichia coli host led to the accumulation of up to 260 mg/L in shake flask cultures. Achievable titers already approach the styrene toxicity threshold (determined as 300 mg/L). To the best of our knowledge, this is the first report of microbial styrene production from sustainable feedstocks.

Original languageEnglish (US)
Title of host publicationInternational Congress on Energy 2011 - Topical Conference at the 2011 AIChE Annual Meeting
PublisherAIChE
Pages672-682
Number of pages11
Volume1
ISBN (Print)9781618395825
StatePublished - Jan 1 2011
EventInternational Congress on Energy 2011 - Topical Conference at the 2011 AIChE Annual Meeting - Minneapolis, United States
Duration: Oct 16 2011Oct 21 2011

Other

OtherInternational Congress on Energy 2011 - Topical Conference at the 2011 AIChE Annual Meeting
CountryUnited States
CityMinneapolis
Period10/16/1110/21/11

Fingerprint

Styrene
Biosynthesis
Escherichia coli
Glucose
commodity
candidacy
Phenylalanine
Yeast
Cinnamates
Isoenzymes
Phenylalanine Ammonia-Lyase
Carboxy-Lyases
Petrochemicals
Feedstocks
Toxicity
Ammonia
Polymers
Monomers
Substrates

Keywords

  • Aromatic
  • Cinnamic acid
  • E. coli
  • L-Phenylalanine
  • Phenylalanine ammonia lyase
  • Styrene

ASJC Scopus subject areas

  • Chemical Health and Safety
  • Safety, Risk, Reliability and Quality
  • Safety Research

Cite this

McKenna, R., & Nielsen, D. (2011). Styrene biosynthesis from glucose by engineered E. Coli. In International Congress on Energy 2011 - Topical Conference at the 2011 AIChE Annual Meeting (Vol. 1, pp. 672-682). AIChE.

Styrene biosynthesis from glucose by engineered E. Coli. / McKenna, Rebekah; Nielsen, David.

International Congress on Energy 2011 - Topical Conference at the 2011 AIChE Annual Meeting. Vol. 1 AIChE, 2011. p. 672-682.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

McKenna, R & Nielsen, D 2011, Styrene biosynthesis from glucose by engineered E. Coli. in International Congress on Energy 2011 - Topical Conference at the 2011 AIChE Annual Meeting. vol. 1, AIChE, pp. 672-682, International Congress on Energy 2011 - Topical Conference at the 2011 AIChE Annual Meeting, Minneapolis, United States, 10/16/11.
McKenna R, Nielsen D. Styrene biosynthesis from glucose by engineered E. Coli. In International Congress on Energy 2011 - Topical Conference at the 2011 AIChE Annual Meeting. Vol. 1. AIChE. 2011. p. 672-682
McKenna, Rebekah ; Nielsen, David. / Styrene biosynthesis from glucose by engineered E. Coli. International Congress on Energy 2011 - Topical Conference at the 2011 AIChE Annual Meeting. Vol. 1 AIChE, 2011. pp. 672-682
@inproceedings{6ef454d3e4fe4c09962d64a3846795a9,
title = "Styrene biosynthesis from glucose by engineered E. Coli",
abstract = "Styrene is a large volume, commodity petrochemical with diverse commercial applications, including as a monomer building-block for the synthesis of many useful polymers. Here we demonstrate how, through the de novo design and development of a novel metabolic pathway, styrene can alternatively be synthesized from renewable substrates such as glucose. The conversion of endogenously synthesized L-phenylalanine to styrene was achieved by the co-expression of phenylalanine ammonia lyase and trans-cinnamate decarboxylase. Candidate isoenzymes for each step were screened from bacterial, yeast, and plant genetic sources. Finally, over-expression of PAL2 from Arabidopsis thaliana and FDC1 from Saccharomyces cerevisiae (originally classified as ferulate decarboxylase) in an L-phenylalanine over-producing Escherichia coli host led to the accumulation of up to 260 mg/L in shake flask cultures. Achievable titers already approach the styrene toxicity threshold (determined as 300 mg/L). To the best of our knowledge, this is the first report of microbial styrene production from sustainable feedstocks.",
keywords = "Aromatic, Cinnamic acid, E. coli, L-Phenylalanine, Phenylalanine ammonia lyase, Styrene",
author = "Rebekah McKenna and David Nielsen",
year = "2011",
month = "1",
day = "1",
language = "English (US)",
isbn = "9781618395825",
volume = "1",
pages = "672--682",
booktitle = "International Congress on Energy 2011 - Topical Conference at the 2011 AIChE Annual Meeting",
publisher = "AIChE",

}

TY - GEN

T1 - Styrene biosynthesis from glucose by engineered E. Coli

AU - McKenna, Rebekah

AU - Nielsen, David

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Styrene is a large volume, commodity petrochemical with diverse commercial applications, including as a monomer building-block for the synthesis of many useful polymers. Here we demonstrate how, through the de novo design and development of a novel metabolic pathway, styrene can alternatively be synthesized from renewable substrates such as glucose. The conversion of endogenously synthesized L-phenylalanine to styrene was achieved by the co-expression of phenylalanine ammonia lyase and trans-cinnamate decarboxylase. Candidate isoenzymes for each step were screened from bacterial, yeast, and plant genetic sources. Finally, over-expression of PAL2 from Arabidopsis thaliana and FDC1 from Saccharomyces cerevisiae (originally classified as ferulate decarboxylase) in an L-phenylalanine over-producing Escherichia coli host led to the accumulation of up to 260 mg/L in shake flask cultures. Achievable titers already approach the styrene toxicity threshold (determined as 300 mg/L). To the best of our knowledge, this is the first report of microbial styrene production from sustainable feedstocks.

AB - Styrene is a large volume, commodity petrochemical with diverse commercial applications, including as a monomer building-block for the synthesis of many useful polymers. Here we demonstrate how, through the de novo design and development of a novel metabolic pathway, styrene can alternatively be synthesized from renewable substrates such as glucose. The conversion of endogenously synthesized L-phenylalanine to styrene was achieved by the co-expression of phenylalanine ammonia lyase and trans-cinnamate decarboxylase. Candidate isoenzymes for each step were screened from bacterial, yeast, and plant genetic sources. Finally, over-expression of PAL2 from Arabidopsis thaliana and FDC1 from Saccharomyces cerevisiae (originally classified as ferulate decarboxylase) in an L-phenylalanine over-producing Escherichia coli host led to the accumulation of up to 260 mg/L in shake flask cultures. Achievable titers already approach the styrene toxicity threshold (determined as 300 mg/L). To the best of our knowledge, this is the first report of microbial styrene production from sustainable feedstocks.

KW - Aromatic

KW - Cinnamic acid

KW - E. coli

KW - L-Phenylalanine

KW - Phenylalanine ammonia lyase

KW - Styrene

UR - http://www.scopus.com/inward/record.url?scp=85054588970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054588970&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85054588970

SN - 9781618395825

VL - 1

SP - 672

EP - 682

BT - International Congress on Energy 2011 - Topical Conference at the 2011 AIChE Annual Meeting

PB - AIChE

ER -