Styrene biosynthesis from glucose by engineered E. coli

Rebekah McKenna, David R. Nielsen

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Styrene is a large volume, commodity petrochemical with diverse commercial applications, including as a monomer building-block for the synthesis of many useful polymers. Here we demonstrate how, through the de novo design and development of a novel metabolic pathway, styrene can alternatively be synthesized from renewable substrates such as glucose. The conversion of endogenously synthesized l-phenylalanine to styrene was achieved by the co-expression of phenylalanine ammonia lyase and trans-cinnamate decarboxylase. Candidate isoenzymes for each step were screened from bacterial, yeast, and plant genetic sources. Finally, over-expression of PAL2 from Arabidopsis thaliana and FDC1 from Saccharomyces cerevisiae (originally classified as ferulate decarboxylase) in an l-phenylalanine over-producing Escherichia coli host led to the accumulation of up to 260. mg/L in shake flask cultures. Achievable titers already approach the styrene toxicity threshold (determined as ∼300. mg/L). To the best of our knowledge, this is the first report of microbial styrene production from sustainable feedstocks.

Original languageEnglish (US)
Pages (from-to)544-554
Number of pages11
JournalMetabolic Engineering
Volume13
Issue number5
DOIs
StatePublished - Sep 2011

Fingerprint

Styrene
Biosynthesis
Escherichia coli
Glucose
Phenylalanine
Yeast
Cinnamates
Isoenzymes
Phenylalanine Ammonia-Lyase
Batch Cell Culture Techniques
Carboxy-Lyases
Metabolic Networks and Pathways
Arabidopsis
Petrochemicals
Feedstocks
Toxicity
Saccharomyces cerevisiae
Ammonia
Polymers
Yeasts

Keywords

  • Aromatic
  • Cinnamic acid
  • E. coli
  • L-Phenylalanine
  • Phenylalanine ammonia lyase
  • Styrene

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Styrene biosynthesis from glucose by engineered E. coli. / McKenna, Rebekah; Nielsen, David R.

In: Metabolic Engineering, Vol. 13, No. 5, 09.2011, p. 544-554.

Research output: Contribution to journalArticle

@article{c77b5666e40b44418d874c9cc30bec41,
title = "Styrene biosynthesis from glucose by engineered E. coli",
abstract = "Styrene is a large volume, commodity petrochemical with diverse commercial applications, including as a monomer building-block for the synthesis of many useful polymers. Here we demonstrate how, through the de novo design and development of a novel metabolic pathway, styrene can alternatively be synthesized from renewable substrates such as glucose. The conversion of endogenously synthesized l-phenylalanine to styrene was achieved by the co-expression of phenylalanine ammonia lyase and trans-cinnamate decarboxylase. Candidate isoenzymes for each step were screened from bacterial, yeast, and plant genetic sources. Finally, over-expression of PAL2 from Arabidopsis thaliana and FDC1 from Saccharomyces cerevisiae (originally classified as ferulate decarboxylase) in an l-phenylalanine over-producing Escherichia coli host led to the accumulation of up to 260. mg/L in shake flask cultures. Achievable titers already approach the styrene toxicity threshold (determined as ∼300. mg/L). To the best of our knowledge, this is the first report of microbial styrene production from sustainable feedstocks.",
keywords = "Aromatic, Cinnamic acid, E. coli, L-Phenylalanine, Phenylalanine ammonia lyase, Styrene",
author = "Rebekah McKenna and Nielsen, {David R.}",
year = "2011",
month = "9",
doi = "10.1016/j.ymben.2011.06.005",
language = "English (US)",
volume = "13",
pages = "544--554",
journal = "Metabolic Engineering",
issn = "1096-7176",
publisher = "Academic Press Inc.",
number = "5",

}

TY - JOUR

T1 - Styrene biosynthesis from glucose by engineered E. coli

AU - McKenna, Rebekah

AU - Nielsen, David R.

PY - 2011/9

Y1 - 2011/9

N2 - Styrene is a large volume, commodity petrochemical with diverse commercial applications, including as a monomer building-block for the synthesis of many useful polymers. Here we demonstrate how, through the de novo design and development of a novel metabolic pathway, styrene can alternatively be synthesized from renewable substrates such as glucose. The conversion of endogenously synthesized l-phenylalanine to styrene was achieved by the co-expression of phenylalanine ammonia lyase and trans-cinnamate decarboxylase. Candidate isoenzymes for each step were screened from bacterial, yeast, and plant genetic sources. Finally, over-expression of PAL2 from Arabidopsis thaliana and FDC1 from Saccharomyces cerevisiae (originally classified as ferulate decarboxylase) in an l-phenylalanine over-producing Escherichia coli host led to the accumulation of up to 260. mg/L in shake flask cultures. Achievable titers already approach the styrene toxicity threshold (determined as ∼300. mg/L). To the best of our knowledge, this is the first report of microbial styrene production from sustainable feedstocks.

AB - Styrene is a large volume, commodity petrochemical with diverse commercial applications, including as a monomer building-block for the synthesis of many useful polymers. Here we demonstrate how, through the de novo design and development of a novel metabolic pathway, styrene can alternatively be synthesized from renewable substrates such as glucose. The conversion of endogenously synthesized l-phenylalanine to styrene was achieved by the co-expression of phenylalanine ammonia lyase and trans-cinnamate decarboxylase. Candidate isoenzymes for each step were screened from bacterial, yeast, and plant genetic sources. Finally, over-expression of PAL2 from Arabidopsis thaliana and FDC1 from Saccharomyces cerevisiae (originally classified as ferulate decarboxylase) in an l-phenylalanine over-producing Escherichia coli host led to the accumulation of up to 260. mg/L in shake flask cultures. Achievable titers already approach the styrene toxicity threshold (determined as ∼300. mg/L). To the best of our knowledge, this is the first report of microbial styrene production from sustainable feedstocks.

KW - Aromatic

KW - Cinnamic acid

KW - E. coli

KW - L-Phenylalanine

KW - Phenylalanine ammonia lyase

KW - Styrene

UR - http://www.scopus.com/inward/record.url?scp=80052027792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052027792&partnerID=8YFLogxK

U2 - 10.1016/j.ymben.2011.06.005

DO - 10.1016/j.ymben.2011.06.005

M3 - Article

VL - 13

SP - 544

EP - 554

JO - Metabolic Engineering

JF - Metabolic Engineering

SN - 1096-7176

IS - 5

ER -