Styrene biosynthesis from glucose by engineered E. coli

Styrene is a large volume, commodity petrochemical with diverse commercial applications, including as a monomer building-block for the synthesis of many use ful polymers. Here we demonstrate how, through the de novo design and development of a novel metabolic pathway, styrene can alternatively be synthesized from renewable substrates such as glucose. The conversion of endogenously synthesized L-phenylalanine to styrene was achieved by the co-expression of phenylalanine ammonia lyase and trans-cinnamate decarboxylase. Candidate isoenzymes for each step were screened from bacterial, yeast,and plant genetic sources. Finally, over-expression of PAL2 from Arabidopsis thaliana and FDC1 from Saccharomyces cerevisiae (originally classified as ferulate decarboxylase) in an L-phenylalanine over-producing Escherichia coli host led to the accumulationof up to 260 mg/L in shake flask cultures. Achievable titers already approach the styrene toxicity threshold (determinedas ∼300 mg/L).To the best of our knowledge, this is the first report of microbial styrene production from sustainable feedstocks.