Study of persistence of enteric viruses in landfilled disposable diapers

M. S. Huber, C. P. Gerba, Morteza Abbaszadegan, J. A. Robinson, S. M. Bradford

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Disposable diapers are one of many possible sources of infectious enteric viruses that are disposed of in landfills. A total of 218 disposable diapers were collected from 7 sites and 10 depths at three landfills. Of this total, 110 diapers were selected to be processed based on fecal content using a 1.5% beef extract elution, organic flocculation-concentration method to recover viruses. The concentrated samples were assayed on Buffalo Green Monkey (BGM) kidney cell cultures for the detection of enteroviruses and with cDNA probes specific for poliovirus, hepatitis A virus, and rotavirus. Enteroviruses were not detected in any sample assayed using cell culture techniques. Three samples were positive using nucleic acid probes for poliovirus while negative for rotavirus and hepatitis A. These results suggest that, although poliovirus RNA was present in some diapers, the viruses were not viable by cell culture assays after 2 years or longer in a landfill.

Original languageEnglish (US)
Pages (from-to)1767-1772
Number of pages6
JournalEnvironmental Science and Technology
Volume28
Issue number9
StatePublished - 1994

Fingerprint

Viruses
virus
persistence
Land fill
Cell culture
landfill
hepatitis
probe
Nucleic Acid Probes
Beef
Nucleic acids
Flocculation
nucleic acid
RNA
flocculation
Assays
Complementary DNA
assay
enterovirus

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Huber, M. S., Gerba, C. P., Abbaszadegan, M., Robinson, J. A., & Bradford, S. M. (1994). Study of persistence of enteric viruses in landfilled disposable diapers. Environmental Science and Technology, 28(9), 1767-1772.

Study of persistence of enteric viruses in landfilled disposable diapers. / Huber, M. S.; Gerba, C. P.; Abbaszadegan, Morteza; Robinson, J. A.; Bradford, S. M.

In: Environmental Science and Technology, Vol. 28, No. 9, 1994, p. 1767-1772.

Research output: Contribution to journalArticle

Huber, MS, Gerba, CP, Abbaszadegan, M, Robinson, JA & Bradford, SM 1994, 'Study of persistence of enteric viruses in landfilled disposable diapers', Environmental Science and Technology, vol. 28, no. 9, pp. 1767-1772.
Huber, M. S. ; Gerba, C. P. ; Abbaszadegan, Morteza ; Robinson, J. A. ; Bradford, S. M. / Study of persistence of enteric viruses in landfilled disposable diapers. In: Environmental Science and Technology. 1994 ; Vol. 28, No. 9. pp. 1767-1772.
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