Structure of the reaction center from Rhodobacter sphaeroides R-26

the protein subunits.

James Allen, G. Feher, T. O. Yeates, H. Komiya, D. C. Rees

Research output: Contribution to journalArticle

528 Citations (Scopus)

Abstract

The three-dimensional structure of the protein subunits of the reaction center (RC) of Rhodobacter sphaeroides has been determined by x-ray diffraction at a resolution of 2.8 A with an R factor of 26%. The L and M subunits each contain five transmembrane helices and several helices that do not span the membrane. The L and M subunits are related to each other by a 2-fold rotational symmetry axis that is approximately the same as that determined for the cofactors. The H subunit has one transmembrane helix and a globular domain on the cytoplasmic side, which contains a helix that does not span the membrane and several beta-sheets. The structural homology with RCs from other purple bacteria is discussed. A structure of the complex formed between the water soluble cytochrome c2 and the RC from Rb. sphaeroides is proposed.

Original languageEnglish (US)
Pages (from-to)6162-6166
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number17
StatePublished - Sep 1987
Externally publishedYes

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Rhodobacter sphaeroides
Protein Subunits
Cytochromes c2
Proteobacteria
Membranes
R Factors
X-Rays
Water
beta-Strand Protein Conformation

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Structure of the reaction center from Rhodobacter sphaeroides R-26 : the protein subunits. / Allen, James; Feher, G.; Yeates, T. O.; Komiya, H.; Rees, D. C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 17, 09.1987, p. 6162-6166.

Research output: Contribution to journalArticle

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