Abstract
Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.
Original language | English (US) |
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Article number | 2911 |
Journal | Nature communications |
Volume | 4 |
DOIs | |
State | Published - Dec 19 2013 |
ASJC Scopus subject areas
- General Chemistry
- General Biochemistry, Genetics and Molecular Biology
- General Physics and Astronomy
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Serial femtosecond crystallography structure of a photosynthetic reaction center
Johansson, L. C. (Contributor), Arnlund, D. (Contributor), Katona, G. (Contributor), White, T. A. (Contributor), Barty, A. (Contributor), Deponte, D. P. (Contributor), Shoeman, R. L. (Contributor), Wickstrand, C. (Contributor), Sharma, A. (Contributor), Williams, G. J. (Contributor), Aquila, A. (Contributor), Bogan, M. J. (Contributor), Caleman, C. (Contributor), Davidsson, J. (Contributor), Doak, R. B. (Contributor), Frank, M. (Contributor), Fromme, R. (Contributor), Galli, L. (Contributor), Grotjohann, I. (Contributor), Hunter, M. S. (Contributor), Kassemeyer, S. (Contributor), Kirian, R. (Contributor), Kupitz, C. (Contributor), Liang, M. (Contributor), Lomb, L. (Contributor), Malmerberg, E. (Contributor), Martin, A. V. (Contributor), Messerschmidt, M. (Contributor), Nass, K. (Contributor), Redecke, L. (Contributor), Seibert, M. M. (Contributor), Sjöhamn, J. (Contributor), Steinbrener, J. (Contributor), Stellato, F. (Contributor), Wang, D. (Contributor), Wahlgren, W. Y. (Contributor), Weierstall, U. (Contributor), Westenhoff, S. (Contributor), Zatsepin, N. A. (Contributor), Boutet, S. (Contributor), Spence, J. C. H. (Contributor), Schlichting, I. (Contributor), Chapman, H. N. (Contributor), Fromme, P. (Contributor) & Neutze, R. (Contributor), Protein Data Bank (PDB), Dec 25 2013
DOI: 10.2210/pdb4CAS, https://www.wwpdb.org/pdb?id=pdb_00004cas
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