Structure of a bacterial 30S ribosomal subunit at 5.5 Å resolution

William M. Clemons, Joanna L.C. May, Brian T. Wimberly, John P. McCutcheon, Malcolm S. Capel, V. Ramakrishnan

Research output: Contribution to journalArticlepeer-review

300 Scopus citations

Abstract

The 30S ribosomal subunit binds messenger RNA and the anticodon stem- loop of transfer RNA during protein synthesis. A crystallographic analysis of the structure of the subunit from the bacterium Thermus thermophilus is presented. At a resolution of 5.5 Å, the phosphate backbone of the ribosomal RNA is visible, as are the α-helices of the ribosomal proteins, enabling double-helical regions of RNA to be identified throughout the subunit, all seven of the small-subunit proteins of known crystal structure to be positioned in the electron density map, and the fold of the entire central domain of the small-subunit ribosomal RNA to be determined.

Original languageEnglish (US)
Pages (from-to)833-840
Number of pages8
JournalNature
Volume400
Issue number6747
DOIs
StatePublished - Aug 26 1999
Externally publishedYes

ASJC Scopus subject areas

  • General

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