Abstract
In this study, we show that maltose-binding protein (MBP) is capable of facilitating stable gold nanoparticle synthesis, and a structure of MBP in the presence of gold ions was determined by X-ray crystallography. Using this high-resolution structure of gold ion bound MBP, a peptide (AT1) was selected and synthesized and was shown to also aid in the synthesis of stable gold nanoparticles under similar experimental conditions to those used for protein facilitated synthesis. This structure-based approach represents a new potential method for the selection of peptides capable of facilitating stable nanoparticle synthesis.
Original language | English (US) |
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Pages (from-to) | 4867-4873 |
Number of pages | 7 |
Journal | Biotechnology and bioengineering |
Volume | 118 |
Issue number | 12 |
DOIs | |
State | Published - Dec 2021 |
Keywords
- biomineralization
- gold nanoparticles
- nanobiotechnology
- protein crystallography
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
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Dive into the research topics of 'Structure-guided identification of a peptide for bio-enabled gold nanoparticle synthesis'. Together they form a unique fingerprint.Datasets
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Tetragonal Maltose Binding Protein in the presence of gold
Thaker, A. (Contributor), Sirajudeen, L. (Contributor), Simmons, C. R. (Contributor) & Nannenga, B. (Contributor), Protein Data Bank (PDB), Sep 8 2021
DOI: 10.2210/pdb7MQ6, https://www.wwpdb.org/pdb?id=pdb_00007mq6
Dataset
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Tetragonal Maltose Binding Protein
Thaker, A. (Contributor), Sirajudeen, L. (Contributor), Simmons, C. R. (Contributor) & Nannenga, B. (Contributor), Protein Data Bank (PDB), Sep 8 2021
DOI: 10.2210/pdb7MQ7, https://www.wwpdb.org/pdb?id=pdb_00007mq7
Dataset