Structure-factor amplitude reconstruction from serial femtosecond crystallography of two-dimensional membrane-protein crystals

Cecilia M. Casadei, Karol Nass, Anton Barty, Mark S. Hunter, Celestino Padeste, Ching Ju Tsai, Sebastien Boutet, Marc Messerschmidt, Leonardo Sala, Garth J. Williams, Dmitry Ozerov, Matthew Coleman, Xiao Dan Li, Matthias Frank, Bill Pedrini

Research output: Contribution to journalArticle

Abstract

Serial femtosecond crystallography of two-dimensional membrane-protein crystals at X-ray free-electron lasers has the potential to address the dynamics of functionally relevant large-scale motions, which can be sterically hindered in three-dimensional crystals and suppressed in cryocooled samples. In previous work, diffraction data limited to a two-dimensional reciprocal-space slice were evaluated and it was demonstrated that the low intensity of the diffraction signal can be overcome by collecting highly redundant data, thus enhancing the achievable resolution. Here, the application of a newly developed method to analyze diffraction data covering three reciprocal-space dimensions, extracting the reciprocal-space map of the structure-factor amplitudes, is presented. Despite the low resolution and completeness of the data set, it is shown by molecular replacement that the reconstructed amplitudes carry meaningful structural information. Therefore, it appears that these intrinsic limitations in resolution and completeness from two-dimensional crystal diffraction may be overcome by collecting highly redundant data along the three reciprocal-space axes, thus allowing the measurement of large-scale dynamics in pump-probe experiments.

Original languageEnglish (US)
Pages (from-to)34-45
Number of pages12
JournalIUCrJ
Volume6
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

Crystallography
crystallography
Membrane Proteins
Lasers
Diffraction
X-Rays
Electrons
membranes
proteins
Proteins
Membranes
Crystals
completeness
diffraction
crystals
X ray lasers
Free electron lasers
free electron lasers
coverings
Pumps

Keywords

  • Free-electron lasers
  • Membrane proteins
  • Serial femto­second crystallography
  • Two-dimensional crystals

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Casadei, C. M., Nass, K., Barty, A., Hunter, M. S., Padeste, C., Tsai, C. J., ... Pedrini, B. (2019). Structure-factor amplitude reconstruction from serial femtosecond crystallography of two-dimensional membrane-protein crystals. IUCrJ, 6, 34-45. https://doi.org/10.1107/S2052252518014641

Structure-factor amplitude reconstruction from serial femtosecond crystallography of two-dimensional membrane-protein crystals. / Casadei, Cecilia M.; Nass, Karol; Barty, Anton; Hunter, Mark S.; Padeste, Celestino; Tsai, Ching Ju; Boutet, Sebastien; Messerschmidt, Marc; Sala, Leonardo; Williams, Garth J.; Ozerov, Dmitry; Coleman, Matthew; Li, Xiao Dan; Frank, Matthias; Pedrini, Bill.

In: IUCrJ, Vol. 6, 01.01.2019, p. 34-45.

Research output: Contribution to journalArticle

Casadei, CM, Nass, K, Barty, A, Hunter, MS, Padeste, C, Tsai, CJ, Boutet, S, Messerschmidt, M, Sala, L, Williams, GJ, Ozerov, D, Coleman, M, Li, XD, Frank, M & Pedrini, B 2019, 'Structure-factor amplitude reconstruction from serial femtosecond crystallography of two-dimensional membrane-protein crystals', IUCrJ, vol. 6, pp. 34-45. https://doi.org/10.1107/S2052252518014641
Casadei, Cecilia M. ; Nass, Karol ; Barty, Anton ; Hunter, Mark S. ; Padeste, Celestino ; Tsai, Ching Ju ; Boutet, Sebastien ; Messerschmidt, Marc ; Sala, Leonardo ; Williams, Garth J. ; Ozerov, Dmitry ; Coleman, Matthew ; Li, Xiao Dan ; Frank, Matthias ; Pedrini, Bill. / Structure-factor amplitude reconstruction from serial femtosecond crystallography of two-dimensional membrane-protein crystals. In: IUCrJ. 2019 ; Vol. 6. pp. 34-45.
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