Structure and thickness dependence of "molecular wiring" in nanostructured enzyme multilayers

Victoria Flexer, Erica Forzani, Ernesto J. Calvo, Silvio J. Ludueña, Lia I. Pietrasanta

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Supramolecular organized multilayers composed of glucose oxidase (GOx) and osmium-derivatized poly(allylamine) redox polymer have been self-assembled electrostatically from Os-polyelectrolyte solutions of variable pH (5.5-8.8) leading to a decrease of the linear charge density in the PAH-Os with increasing pH. The layer-by-layer enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of β-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed.

Original languageEnglish (US)
Pages (from-to)399-407
Number of pages9
JournalAnalytical Chemistry
Volume78
Issue number2
DOIs
StatePublished - Jan 15 2006
Externally publishedYes

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Electric wiring
Multilayers
Polyelectrolytes
Enzymes
Allylamine
Osmium
Adsorption
Oxidation
Glucose Oxidase
Quartz crystal microbalances
Ellipsometry
Film growth
Polycyclic aromatic hydrocarbons
Charge density
Cyclic voltammetry
Film thickness
Rate constants
Polymers
Glucose
Oxidation-Reduction

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Structure and thickness dependence of "molecular wiring" in nanostructured enzyme multilayers. / Flexer, Victoria; Forzani, Erica; Calvo, Ernesto J.; Ludueña, Silvio J.; Pietrasanta, Lia I.

In: Analytical Chemistry, Vol. 78, No. 2, 15.01.2006, p. 399-407.

Research output: Contribution to journalArticle

Flexer, Victoria ; Forzani, Erica ; Calvo, Ernesto J. ; Ludueña, Silvio J. ; Pietrasanta, Lia I. / Structure and thickness dependence of "molecular wiring" in nanostructured enzyme multilayers. In: Analytical Chemistry. 2006 ; Vol. 78, No. 2. pp. 399-407.
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