Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein

T. E. Kienzle, S. Abraham, B. G. Hogue, D. A. Brian

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

The sequence of the hemagglutinin-esterase (HE) gene for the Mebus strain of bovine coronavirus was obtained from cDNA clones, and its deduced product is a 47,700-kilodalton apoprotein of 424 amino acids. Expression of the HE protein in vitro in the presence of microsomes revealed N-terminal signal peptide cleavage and C-terminal anchorage but not disulfide-linked dimerization. Dimerization was observed only after expression in vivo, during which HE was also transported to the cell surface.

Original languageEnglish (US)
Pages (from-to)1834-1838
Number of pages5
JournalJournal of virology
Volume64
Issue number4
StatePublished - Jan 1 1990
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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