Structure and function of the smallest vertebrate telomerase RNA from teleost fish

Mingyi Xie, Axel Mosig, Xiaodong Qi, Yang Li, Peter F. Stadler, Julian Chen

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Telomerase extends chromosome ends by copying a short template sequence within its intrinsic RNA component. Telomerase RNA (TR) from different groups of species varies dramatically in sequence and size. We report here the bioinformatic identification, secondary structure comparison, and functional analysis of the smallest known vertebrate TRs from five teleost fishes. The teleost TRs (312-348 nucleotides) are significantly smaller than the cartilaginous fish TRs (478-559 nucleotides) and tetrapod TRs. This remarkable length reduction of teleost fish TRs correlates positively with the genome size, reflecting an unusual structural plasticity of TR during evolution. The teleost TR consists of a compact three-domain structure, lacking most of the sequences in regions that are variable in other vertebrate TR structures. The medaka and fugu TRs, when assembled with their telomerase reverse transcriptase (TERT) protein counterparts, reconstituted active and processive telomerase enzymes. Titration analysis of individual RNA domains suggests that the efficient assembly of the telomerase complex is influenced more by the telomerase reverse transcriptase (TERT) binding of the CR4-CR5 domain than the pseudoknot domain of TR. The remarkably small teleost fish TR further expands our understanding about the evolutionary divergence of vertebrate TR.

Original languageEnglish (US)
Pages (from-to)2049-2059
Number of pages11
JournalJournal of Biological Chemistry
Volume283
Issue number4
DOIs
StatePublished - Jan 25 2008

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Fish
Vertebrates
Fishes
Telomerase
Nucleotides
Takifugu
RNA
Oryzias
Genome Size
Functional analysis
Copying
Bioinformatics
Chromosomes
telomerase RNA
Computational Biology
Titration
Plasticity
Genes
Enzymes
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structure and function of the smallest vertebrate telomerase RNA from teleost fish. / Xie, Mingyi; Mosig, Axel; Qi, Xiaodong; Li, Yang; Stadler, Peter F.; Chen, Julian.

In: Journal of Biological Chemistry, Vol. 283, No. 4, 25.01.2008, p. 2049-2059.

Research output: Contribution to journalArticle

Xie, Mingyi ; Mosig, Axel ; Qi, Xiaodong ; Li, Yang ; Stadler, Peter F. ; Chen, Julian. / Structure and function of the smallest vertebrate telomerase RNA from teleost fish. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 4. pp. 2049-2059.
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