Structure and function of glucose binding protein-single walled carbon nanotube complexes

Thomas P. McNicholas, Kyungsuk Yum, Jin Ho Ahn, Bin Mu, Oliver Plettenburg, Annlouise Gooderman, Sridaran Natesan, Michael S. Strano

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Understanding the structure and function of glucose binding proteins (GBP) complexed with single walled carbon nanotubes (SWNTs) is important for the development of applications including fluorescent sensors and nanostructure particle tracking. Herein, circular dichroism (CD), thermal denaturation, photo-absorption spectroscopy and atomic force microscopy are used to study these nanostructures. The protein retains its glucose-binding activity after complexation and is thermally stable below 36 °C. However, the SWNT lowers the midpoint denaturation temperature (Tm) by 5°C and 4°C in the absence and presence of 10 mM glucose, respectively. This data highlights that using techniques such as CD and thermal denaturation may be necessary to fully characterize such protein-nanomaterial nanostructures.

Original languageEnglish (US)
Pages (from-to)3510-3516
Number of pages7
JournalSmall
Volume8
Issue number22
DOIs
StatePublished - Nov 19 2012
Externally publishedYes

Fingerprint

Carbon Nanotubes
Denaturation
Nanostructures
Single-walled carbon nanotubes (SWCN)
Glucose
Carrier Proteins
Dichroism
Circular Dichroism
Proteins
Hot Temperature
Complexation
Absorption spectroscopy
Nanostructured materials
Atomic Force Microscopy
Atomic force microscopy
Spectrum Analysis
Sensors
Temperature

Keywords

  • carbon nanotubes
  • fluorescence
  • glucose
  • periplasmic-binding proteins

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology

Cite this

McNicholas, T. P., Yum, K., Ahn, J. H., Mu, B., Plettenburg, O., Gooderman, A., ... Strano, M. S. (2012). Structure and function of glucose binding protein-single walled carbon nanotube complexes. Small, 8(22), 3510-3516. https://doi.org/10.1002/smll.201200649

Structure and function of glucose binding protein-single walled carbon nanotube complexes. / McNicholas, Thomas P.; Yum, Kyungsuk; Ahn, Jin Ho; Mu, Bin; Plettenburg, Oliver; Gooderman, Annlouise; Natesan, Sridaran; Strano, Michael S.

In: Small, Vol. 8, No. 22, 19.11.2012, p. 3510-3516.

Research output: Contribution to journalArticle

McNicholas, TP, Yum, K, Ahn, JH, Mu, B, Plettenburg, O, Gooderman, A, Natesan, S & Strano, MS 2012, 'Structure and function of glucose binding protein-single walled carbon nanotube complexes', Small, vol. 8, no. 22, pp. 3510-3516. https://doi.org/10.1002/smll.201200649
McNicholas TP, Yum K, Ahn JH, Mu B, Plettenburg O, Gooderman A et al. Structure and function of glucose binding protein-single walled carbon nanotube complexes. Small. 2012 Nov 19;8(22):3510-3516. https://doi.org/10.1002/smll.201200649
McNicholas, Thomas P. ; Yum, Kyungsuk ; Ahn, Jin Ho ; Mu, Bin ; Plettenburg, Oliver ; Gooderman, Annlouise ; Natesan, Sridaran ; Strano, Michael S. / Structure and function of glucose binding protein-single walled carbon nanotube complexes. In: Small. 2012 ; Vol. 8, No. 22. pp. 3510-3516.
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