Structurally altered substrates for DNA topoisomerase I. Effects of inclusion of a single 3'-deoxynucleotide within the scissile strand

Tuncer Arslan, Anil T. Abraham, Sidney M. Hecht

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A partial DNA duplex containing a high efficiency topoisomerase I cleavage site was substituted singly at each of three sites with 3'- deoxyadenosine. Depending on the site of substitution, the facility of the topoisomerase I-mediated cleavage or ligation reactions was altered. Inclusion of the modified nucleoside at the 5'-end of the acceptor oligonucleotide diminished the rate of religation following substrate cleavage by the enzyme.

Original languageEnglish (US)
Pages (from-to)515-530
Number of pages16
JournalNucleosides and Nucleotides
Volume17
Issue number1-3
StatePublished - Apr 8 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

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