Structurally altered substrates for DNA topoisomerase I. Effects of inclusion of a single 3'-deoxynucleotide within the scissile strand

Tuncer Arslan; Anil T. Abraham; Sidney M. Hecht

doi:10.1080/07328319808005195

Structurally altered substrates for DNA topoisomerase I. Effects of inclusion of a single 3'-deoxynucleotide within the scissile strand

Tuncer Arslan, Anil T. Abraham, Sidney M. Hecht

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A partial DNA duplex containing a high efficiency topoisomerase I cleavage site was substituted singly at each of three sites with 3'- deoxyadenosine. Depending on the site of substitution, the facility of the topoisomerase I-mediated cleavage or ligation reactions was altered. Inclusion of the modified nucleoside at the 5'-end of the acceptor oligonucleotide diminished the rate of religation following substrate cleavage by the enzyme.

Original language	English (US)
Pages (from-to)	515-530
Number of pages	16
Journal	Nucleosides and Nucleotides
Volume	17
Issue number	1-3
DOIs	https://doi.org/10.1080/07328319808005195
State	Published - 1998
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Genetics

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10.1080/07328319808005195

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abstract = "A partial DNA duplex containing a high efficiency topoisomerase I cleavage site was substituted singly at each of three sites with 3'- deoxyadenosine. Depending on the site of substitution, the facility of the topoisomerase I-mediated cleavage or ligation reactions was altered. Inclusion of the modified nucleoside at the 5'-end of the acceptor oligonucleotide diminished the rate of religation following substrate cleavage by the enzyme.",

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Structurally altered substrates for DNA topoisomerase I. Effects of inclusion of a single 3'-deoxynucleotide within the scissile strand

Abstract

ASJC Scopus subject areas

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