Structural Insights into Conformation Differences between DNA/TNA and RNA/TNA Chimeric Duplexes

Irina Anosova, Ewa A. Kowal, Nicholas J. Sisco, Sujay Sau, Jen Yu Liao, Saikat Bala, Eriks Rozners, Martin Egli, John C. Chaput, Wade Van Horn

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Threose nucleic acid (TNA) is an artificial genetic polymer capable of heredity and evolution, and is studied in the context of RNA chemical etiology. It has a four-carbon threose backbone in place of the five-carbon ribose of natural nucleic acids, yet forms stable antiparallel complementary Watson–Crick homoduplexes and heteroduplexes with DNA and RNA. TNA base-pairs more favorably with RNA than with DNA but the reason is unknown. Here, we employed NMR, ITC, UV, and CD to probe the structural and dynamic properties of heteroduplexes of RNA/TNA and DNA/TNA. The results indicate that TNA templates the structure of heteroduplexes, thereby forcing an A-like helical geometry. NMR measurement of kinetic and thermodynamic parameters for individual base pair opening events reveal unexpected asymmetric “breathing” fluctuations of the DNA/TNA helix. The results suggest that DNA is unable to fully adapt to the conformational constraints of the rigid TNA backbone and that nucleic acid breathing dynamics are determined from both backbone and base contributions.

Original languageEnglish (US)
Pages (from-to)1705-1708
Number of pages4
JournalChemBioChem
Volume17
Issue number18
DOIs
StatePublished - Sep 15 2016

Fingerprint

Nucleic Acids
Conformations
RNA
DNA
Base Pairing
Respiration
Carbon
Nucleic Acid Heteroduplexes
Nuclear magnetic resonance
erythrose
Heredity
Ribose
Thermodynamics
Polymers
Kinetics
Geometry

Keywords

  • conformation analysis
  • DNA
  • nucleic acid dynamics
  • RNA evolution
  • TNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

Cite this

Structural Insights into Conformation Differences between DNA/TNA and RNA/TNA Chimeric Duplexes. / Anosova, Irina; Kowal, Ewa A.; Sisco, Nicholas J.; Sau, Sujay; Liao, Jen Yu; Bala, Saikat; Rozners, Eriks; Egli, Martin; Chaput, John C.; Van Horn, Wade.

In: ChemBioChem, Vol. 17, No. 18, 15.09.2016, p. 1705-1708.

Research output: Contribution to journalArticle

Anosova, I, Kowal, EA, Sisco, NJ, Sau, S, Liao, JY, Bala, S, Rozners, E, Egli, M, Chaput, JC & Van Horn, W 2016, 'Structural Insights into Conformation Differences between DNA/TNA and RNA/TNA Chimeric Duplexes', ChemBioChem, vol. 17, no. 18, pp. 1705-1708. https://doi.org/10.1002/cbic.201600349
Anosova, Irina ; Kowal, Ewa A. ; Sisco, Nicholas J. ; Sau, Sujay ; Liao, Jen Yu ; Bala, Saikat ; Rozners, Eriks ; Egli, Martin ; Chaput, John C. ; Van Horn, Wade. / Structural Insights into Conformation Differences between DNA/TNA and RNA/TNA Chimeric Duplexes. In: ChemBioChem. 2016 ; Vol. 17, No. 18. pp. 1705-1708.
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