Structural homology of reaction centers from Rhodopseudomonas sphaeroides and Rhodopseudomonas viridis as determined by x-ray diffraction

James Allen, G. Feher, T. O. Yeates, D. C. Rees, J. Deisenhofer, H. Michel, R. Huber

Research output: Contribution to journalArticle

311 Citations (Scopus)

Abstract

Crystals of the reaction center (RC) from Rhodopseudomonas sphaeroides with the space group P212121, have been studied by x-ray diffraction. The Patterson search (molecular replacement) technique was used to analyze the data, with the structure of the reaction center from Rhodopseudomonas viridis as a model system. A preliminary electron density map of the reaction center from R. sphaeroides has been obtained. Comparison of the structure of the RC from R. sphaeroides with that from R. viridis showed the following conserved features: five membrane-spanning helices in each of the L and M subunits, a single membrane-spanning helix in the H subunit, a 2-fold symmetry axis, and similar positions and orientations of the cofactors. Unlike the RCs from R. viridis, both quinones are retained in the RCs from R. sphaeroides. The secondary quinone is located near the position related by the 2-fold symmetry axis to the primary quinone.

Original languageEnglish (US)
Pages (from-to)8589-8593
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number22
DOIs
StatePublished - 1986
Externally publishedYes

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Rhodopseudomonas
Rhodobacter sphaeroides
X-Rays
Quinones
Membranes
Electrons
benzoquinone

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Structural homology of reaction centers from Rhodopseudomonas sphaeroides and Rhodopseudomonas viridis as determined by x-ray diffraction. / Allen, James; Feher, G.; Yeates, T. O.; Rees, D. C.; Deisenhofer, J.; Michel, H.; Huber, R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 22, 1986, p. 8589-8593.

Research output: Contribution to journalArticle

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abstract = "Crystals of the reaction center (RC) from Rhodopseudomonas sphaeroides with the space group P212121, have been studied by x-ray diffraction. The Patterson search (molecular replacement) technique was used to analyze the data, with the structure of the reaction center from Rhodopseudomonas viridis as a model system. A preliminary electron density map of the reaction center from R. sphaeroides has been obtained. Comparison of the structure of the RC from R. sphaeroides with that from R. viridis showed the following conserved features: five membrane-spanning helices in each of the L and M subunits, a single membrane-spanning helix in the H subunit, a 2-fold symmetry axis, and similar positions and orientations of the cofactors. Unlike the RCs from R. viridis, both quinones are retained in the RCs from R. sphaeroides. The secondary quinone is located near the position related by the 2-fold symmetry axis to the primary quinone.",
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AU - Allen, James

AU - Feher, G.

AU - Yeates, T. O.

AU - Rees, D. C.

AU - Deisenhofer, J.

AU - Michel, H.

AU - Huber, R.

PY - 1986

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AB - Crystals of the reaction center (RC) from Rhodopseudomonas sphaeroides with the space group P212121, have been studied by x-ray diffraction. The Patterson search (molecular replacement) technique was used to analyze the data, with the structure of the reaction center from Rhodopseudomonas viridis as a model system. A preliminary electron density map of the reaction center from R. sphaeroides has been obtained. Comparison of the structure of the RC from R. sphaeroides with that from R. viridis showed the following conserved features: five membrane-spanning helices in each of the L and M subunits, a single membrane-spanning helix in the H subunit, a 2-fold symmetry axis, and similar positions and orientations of the cofactors. Unlike the RCs from R. viridis, both quinones are retained in the RCs from R. sphaeroides. The secondary quinone is located near the position related by the 2-fold symmetry axis to the primary quinone.

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