TY - JOUR
T1 - Structural homology of reaction centers from Rhodopseudomonas sphaeroides and Rhodopseudomonas viridis as determined by x-ray diffraction
AU - Allen, J. P.
AU - Feher, G.
AU - Yeates, T. O.
AU - Rees, D. C.
AU - Deisenhofer, J.
AU - Michel, H.
AU - Huber, R.
PY - 1986
Y1 - 1986
N2 - Crystals of the reaction center (RC) from Rhodopseudomonas sphaeroides with the space group P212121, have been studied by x-ray diffraction. The Patterson search (molecular replacement) technique was used to analyze the data, with the structure of the reaction center from Rhodopseudomonas viridis as a model system. A preliminary electron density map of the reaction center from R. sphaeroides has been obtained. Comparison of the structure of the RC from R. sphaeroides with that from R. viridis showed the following conserved features: five membrane-spanning helices in each of the L and M subunits, a single membrane-spanning helix in the H subunit, a 2-fold symmetry axis, and similar positions and orientations of the cofactors. Unlike the RCs from R. viridis, both quinones are retained in the RCs from R. sphaeroides. The secondary quinone is located near the position related by the 2-fold symmetry axis to the primary quinone.
AB - Crystals of the reaction center (RC) from Rhodopseudomonas sphaeroides with the space group P212121, have been studied by x-ray diffraction. The Patterson search (molecular replacement) technique was used to analyze the data, with the structure of the reaction center from Rhodopseudomonas viridis as a model system. A preliminary electron density map of the reaction center from R. sphaeroides has been obtained. Comparison of the structure of the RC from R. sphaeroides with that from R. viridis showed the following conserved features: five membrane-spanning helices in each of the L and M subunits, a single membrane-spanning helix in the H subunit, a 2-fold symmetry axis, and similar positions and orientations of the cofactors. Unlike the RCs from R. viridis, both quinones are retained in the RCs from R. sphaeroides. The secondary quinone is located near the position related by the 2-fold symmetry axis to the primary quinone.
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U2 - 10.1073/pnas.83.22.8589
DO - 10.1073/pnas.83.22.8589
M3 - Article
C2 - 3022298
AN - SCOPUS:0000840344
SN - 0027-8424
VL - 83
SP - 8589
EP - 8593
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 22
ER -