Structural genomics of pathogenic protozoa: an overview.

Erkang Fan, David Baker, Stanley Fields, Michael H. Gelb, Frederick S. Buckner, Wesley C. Van Voorhis, Eric Phizicky, Mark Dumont, Christopher Mehlin, Elizabeth Grayhack, Mark Sullivan, Christophe Verlinde, George Detitta, Deirdre Meldrum, Ethan A. Merritt, Thomas Earnest, Michael Soltis, Frank Zucker, Peter J. Myler, Lori SchoenfeldDavid Kim, Liz Worthey, Doug Lacount, Marissa Vignali, Jizhen Li, Somnath Mondal, Archna Massey, Brian Carroll, Stacey Gulde, Joseph Luft, Larry Desoto, Mark Holl, Jonathan Caruthers, Jürgen Bosch, Mark Robien, Tracy Arakaki, Margaret Holmes, Isolde Le Trong, Wim G J Hol

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.

Original languageEnglish (US)
Pages (from-to)497-513
Number of pages17
JournalMethods in molecular biology (Clifton, N.J.)
Volume426
StatePublished - 2008
Externally publishedYes

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Genomics
Crystallization
Crystallography
Malaria
Parasites
Proteins
Industrial Development

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Fan, E., Baker, D., Fields, S., Gelb, M. H., Buckner, F. S., Van Voorhis, W. C., ... Hol, W. G. J. (2008). Structural genomics of pathogenic protozoa: an overview. Methods in molecular biology (Clifton, N.J.), 426, 497-513.

Structural genomics of pathogenic protozoa : an overview. / Fan, Erkang; Baker, David; Fields, Stanley; Gelb, Michael H.; Buckner, Frederick S.; Van Voorhis, Wesley C.; Phizicky, Eric; Dumont, Mark; Mehlin, Christopher; Grayhack, Elizabeth; Sullivan, Mark; Verlinde, Christophe; Detitta, George; Meldrum, Deirdre; Merritt, Ethan A.; Earnest, Thomas; Soltis, Michael; Zucker, Frank; Myler, Peter J.; Schoenfeld, Lori; Kim, David; Worthey, Liz; Lacount, Doug; Vignali, Marissa; Li, Jizhen; Mondal, Somnath; Massey, Archna; Carroll, Brian; Gulde, Stacey; Luft, Joseph; Desoto, Larry; Holl, Mark; Caruthers, Jonathan; Bosch, Jürgen; Robien, Mark; Arakaki, Tracy; Holmes, Margaret; Le Trong, Isolde; Hol, Wim G J.

In: Methods in molecular biology (Clifton, N.J.), Vol. 426, 2008, p. 497-513.

Research output: Contribution to journalArticle

Fan, E, Baker, D, Fields, S, Gelb, MH, Buckner, FS, Van Voorhis, WC, Phizicky, E, Dumont, M, Mehlin, C, Grayhack, E, Sullivan, M, Verlinde, C, Detitta, G, Meldrum, D, Merritt, EA, Earnest, T, Soltis, M, Zucker, F, Myler, PJ, Schoenfeld, L, Kim, D, Worthey, L, Lacount, D, Vignali, M, Li, J, Mondal, S, Massey, A, Carroll, B, Gulde, S, Luft, J, Desoto, L, Holl, M, Caruthers, J, Bosch, J, Robien, M, Arakaki, T, Holmes, M, Le Trong, I & Hol, WGJ 2008, 'Structural genomics of pathogenic protozoa: an overview.', Methods in molecular biology (Clifton, N.J.), vol. 426, pp. 497-513.
Fan E, Baker D, Fields S, Gelb MH, Buckner FS, Van Voorhis WC et al. Structural genomics of pathogenic protozoa: an overview. Methods in molecular biology (Clifton, N.J.). 2008;426:497-513.
Fan, Erkang ; Baker, David ; Fields, Stanley ; Gelb, Michael H. ; Buckner, Frederick S. ; Van Voorhis, Wesley C. ; Phizicky, Eric ; Dumont, Mark ; Mehlin, Christopher ; Grayhack, Elizabeth ; Sullivan, Mark ; Verlinde, Christophe ; Detitta, George ; Meldrum, Deirdre ; Merritt, Ethan A. ; Earnest, Thomas ; Soltis, Michael ; Zucker, Frank ; Myler, Peter J. ; Schoenfeld, Lori ; Kim, David ; Worthey, Liz ; Lacount, Doug ; Vignali, Marissa ; Li, Jizhen ; Mondal, Somnath ; Massey, Archna ; Carroll, Brian ; Gulde, Stacey ; Luft, Joseph ; Desoto, Larry ; Holl, Mark ; Caruthers, Jonathan ; Bosch, Jürgen ; Robien, Mark ; Arakaki, Tracy ; Holmes, Margaret ; Le Trong, Isolde ; Hol, Wim G J. / Structural genomics of pathogenic protozoa : an overview. In: Methods in molecular biology (Clifton, N.J.). 2008 ; Vol. 426. pp. 497-513.
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