Structural genomics of pathogenic protozoa: an overview.

Erkang Fan; David Baker; Stanley Fields; Michael H. Gelb; Frederick S. Buckner; Wesley C. Van Voorhis; Eric Phizicky; Mark Dumont; Christopher Mehlin; Elizabeth Grayhack; Mark Sullivan; Christophe Verlinde; George Detitta; Deirdre R. Meldrum; Ethan A. Merritt; Thomas Earnest; Michael Soltis; Frank Zucker; Peter J. Myler; Lori Schoenfeld; David Kim; Liz Worthey; Doug Lacount; Marissa Vignali; Jizhen Li; Somnath Mondal; Archna Massey; Brian Carroll; Stacey Gulde; Joseph Luft; Larry Desoto; Mark Holl; Jonathan Caruthers; Jürgen Bosch; Mark Robien; Tracy Arakaki; Margaret Holmes; Isolde Le Trong; Wim G.J. Hol

doi:10.1007/978-1-60327-058-8_33

Structural genomics of pathogenic protozoa: an overview.

Erkang Fan, David Baker, Stanley Fields, Michael H. Gelb, Frederick S. Buckner, Wesley C. Van Voorhis, Eric Phizicky, Mark Dumont, Christopher Mehlin, Elizabeth Grayhack, Mark Sullivan, Christophe Verlinde, George Detitta, Deirdre R. Meldrum, Ethan A. Merritt, Thomas Earnest, Michael Soltis, Frank Zucker, Peter J. Myler, Lori SchoenfeldDavid Kim, Liz Worthey, Doug Lacount, Marissa Vignali, Jizhen Li, Somnath Mondal, Archna Massey, Brian Carroll, Stacey Gulde, Joseph Luft, Larry Desoto, Mark Holl, Jonathan Caruthers, Jürgen Bosch, Mark Robien, Tracy Arakaki, Margaret Holmes, Isolde Le Trong, Wim G.J. Hol

Research output: Contribution to journal › Review article › peer-review

41 Scopus citations

Abstract

The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.

Original language	English (US)
Pages (from-to)	497-513
Number of pages	17
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	426
DOIs	https://doi.org/10.1007/978-1-60327-058-8_33
State	Published - 2008
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics

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Fan, E., Baker, D., Fields, S., Gelb, M. H., Buckner, F. S., Van Voorhis, W. C., Phizicky, E., Dumont, M., Mehlin, C., Grayhack, E., Sullivan, M., Verlinde, C., Detitta, G., Meldrum, D. R., Merritt, E. A., Earnest, T., Soltis, M., Zucker, F., Myler, P. J., ... Hol, W. G. J. (2008). Structural genomics of pathogenic protozoa: an overview. Methods in molecular biology (Clifton, N.J.), 426, 497-513. https://doi.org/10.1007/978-1-60327-058-8_33

Fan, E, Baker, D, Fields, S, Gelb, MH, Buckner, FS, Van Voorhis, WC, Phizicky, E, Dumont, M, Mehlin, C, Grayhack, E, Sullivan, M, Verlinde, C, Detitta, G, Meldrum, DR, Merritt, EA, Earnest, T, Soltis, M, Zucker, F, Myler, PJ, Schoenfeld, L, Kim, D, Worthey, L, Lacount, D, Vignali, M, Li, J, Mondal, S, Massey, A, Carroll, B, Gulde, S, Luft, J, Desoto, L, Holl, M, Caruthers, J, Bosch, J, Robien, M, Arakaki, T, Holmes, M, Le Trong, I & Hol, WGJ 2008, 'Structural genomics of pathogenic protozoa: an overview.', Methods in molecular biology (Clifton, N.J.), vol. 426, pp. 497-513. https://doi.org/10.1007/978-1-60327-058-8_33

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abstract = "The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of {"}co-crystallants,{"} and capillary crystallization. {"}Fragment cocktail crystallography{"} for medical structural genomics is also described.",

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doi = "10.1007/978-1-60327-058-8_33",

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T2 - an overview.

AU - Fan, Erkang

AU - Baker, David

AU - Fields, Stanley

AU - Gelb, Michael H.

AU - Buckner, Frederick S.

AU - Van Voorhis, Wesley C.

AU - Phizicky, Eric

AU - Dumont, Mark

AU - Mehlin, Christopher

AU - Grayhack, Elizabeth

AU - Sullivan, Mark

AU - Verlinde, Christophe

AU - Detitta, George

AU - Meldrum, Deirdre R.

AU - Merritt, Ethan A.

AU - Earnest, Thomas

AU - Soltis, Michael

AU - Zucker, Frank

AU - Myler, Peter J.

AU - Schoenfeld, Lori

AU - Kim, David

AU - Worthey, Liz

AU - Lacount, Doug

AU - Vignali, Marissa

AU - Li, Jizhen

AU - Mondal, Somnath

AU - Massey, Archna

AU - Carroll, Brian

AU - Gulde, Stacey

AU - Luft, Joseph

AU - Desoto, Larry

AU - Holl, Mark

AU - Caruthers, Jonathan

AU - Bosch, Jürgen

AU - Robien, Mark

AU - Arakaki, Tracy

AU - Holmes, Margaret

AU - Le Trong, Isolde

AU - Hol, Wim G.J.

PY - 2008

Y1 - 2008

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AB - The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.

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AN - SCOPUS:47349086162

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JO - Methods in molecular biology (Clifton, N.J.)

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ER -

Structural genomics of pathogenic protozoa: an overview.

Abstract

ASJC Scopus subject areas

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