Structural genomics of pathogenic protozoa: an overview.

Erkang Fan, David Baker, Stanley Fields, Michael H. Gelb, Frederick S. Buckner, Wesley C. Van Voorhis, Eric Phizicky, Mark Dumont, Christopher Mehlin, Elizabeth Grayhack, Mark Sullivan, Christophe Verlinde, George Detitta, Deirdre R. Meldrum, Ethan A. Merritt, Thomas Earnest, Michael Soltis, Frank Zucker, Peter J. Myler, Lori SchoenfeldDavid Kim, Liz Worthey, Doug Lacount, Marissa Vignali, Jizhen Li, Somnath Mondal, Archna Massey, Brian Carroll, Stacey Gulde, Joseph Luft, Larry Desoto, Mark Holl, Jonathan Caruthers, Jürgen Bosch, Mark Robien, Tracy Arakaki, Margaret Holmes, Isolde Le Trong, Wim G.J. Hol

Research output: Contribution to journalReview article

34 Scopus citations

Abstract

The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.

Original languageEnglish (US)
Pages (from-to)497-513
Number of pages17
JournalMethods in molecular biology (Clifton, N.J.)
Volume426
DOIs
StatePublished - 2008

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Structural genomics of pathogenic protozoa: an overview.'. Together they form a unique fingerprint.

  • Cite this

    Fan, E., Baker, D., Fields, S., Gelb, M. H., Buckner, F. S., Van Voorhis, W. C., Phizicky, E., Dumont, M., Mehlin, C., Grayhack, E., Sullivan, M., Verlinde, C., Detitta, G., Meldrum, D. R., Merritt, E. A., Earnest, T., Soltis, M., Zucker, F., Myler, P. J., ... Hol, W. G. J. (2008). Structural genomics of pathogenic protozoa: an overview. Methods in molecular biology (Clifton, N.J.), 426, 497-513. https://doi.org/10.1007/978-1-60327-058-8_33