Structural characterization of plant-derived hepatitis B surface antigen employed in oral immunization studies

Mark L. Smith, Lizabeth Richter, Charles J. Arntzen, Michael L. Shuler, Hugh Mason

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Several subunit vaccine antigens have been successfully expressed in plants and recently the hepatitis B surface antigen (HBsAg), expressed in potatoes, was shown to be orally immunogenic in animal studies. However, to date, a detailed analysis of the plant-derived antigen is lacking. Herein, we comprehensively characterize the structure and post-translational processing of HBsAg from potato tuber and two plant cell suspension cultures. The HBsAg was found to accumulate intracellularly as tubular structures, with a complex size distribution, differing substantially from the virus-like particle (VLP) preparations of the current commercial vaccines. Extensive disulfide-bond cross-linking, which is important for immunogenicity, was evident and 21-37% of total HBsAg protein displayed epitopes which correlate with vaccine potency. The significance of these results with regard to the production of cost-effective orally delivered vaccines is discussed.

Original languageEnglish (US)
Pages (from-to)4011-4021
Number of pages11
JournalVaccine
Volume21
Issue number25-26
DOIs
StatePublished - Aug 2003

Fingerprint

oral vaccination
Hepatitis B Surface Antigens
Immunization
vaccines
Solanum tuberosum
Plant Antigens
Vaccines
Vaccine Potency
potatoes
antigens
Subunit Vaccines
virus-like particles
subunit vaccines
disulfide bonds
Plant Cells
crosslinking
production costs
Disulfides
Virion
cell suspension culture

Keywords

  • Antigen structure
  • Edible vaccine
  • Hepatitis B surface antigen

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Structural characterization of plant-derived hepatitis B surface antigen employed in oral immunization studies. / Smith, Mark L.; Richter, Lizabeth; Arntzen, Charles J.; Shuler, Michael L.; Mason, Hugh.

In: Vaccine, Vol. 21, No. 25-26, 08.2003, p. 4011-4021.

Research output: Contribution to journalArticle

Smith, Mark L. ; Richter, Lizabeth ; Arntzen, Charles J. ; Shuler, Michael L. ; Mason, Hugh. / Structural characterization of plant-derived hepatitis B surface antigen employed in oral immunization studies. In: Vaccine. 2003 ; Vol. 21, No. 25-26. pp. 4011-4021.
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