Abstract
In higher plants, chloroplast ATP synthase has a unique redox switch on its γ subunit that modulates enzyme activity to limit ATP hydrolysis at night. To understand the molecular details of the redox modulation, we used single-particle cryo-EM to determine the structures of spinach chloroplast ATP synthase in both reduced and oxidized states. The disulfide linkage of the oxidized γ subunit introduces a torsional constraint to stabilize the two β hairpin structures. Once reduced, free cysteines alleviate this constraint, resulting in a concerted motion of the enzyme complex and a smooth transition between rotary states to facilitate the ATP synthesis. We added an uncompetitive inhibitor, tentoxin, in the reduced sample to limit the flexibility of the enzyme and obtained high-resolution details. Our cryo-EM structures provide mechanistic insight into the redox modulation of the energy regulation activity of chloroplast ATP synthase.
Original language | English (US) |
---|---|
Article number | 482 |
Journal | Communications Biology |
Volume | 3 |
Issue number | 1 |
DOIs | |
State | Published - Dec 1 2020 |
ASJC Scopus subject areas
- Medicine (miscellaneous)
- General Biochemistry, Genetics and Molecular Biology
- General Agricultural and Biological Sciences
Fingerprint
Dive into the research topics of 'Structural basis of redox modulation on chloroplast ATP synthase'. Together they form a unique fingerprint.Datasets
-
Chloroplast ATP synthase (R3, CF1FO)
Yang, J. (Contributor), Williams, D. (Contributor), Kandiah, E. (Contributor), Fromme, P. (Contributor) & Chiu, P. (Contributor), Protein Data Bank (PDB), Sep 9 2020
DOI: 10.2210/pdb6VOJ, https://www.wwpdb.org/pdb?id=pdb_00006voj
Dataset
-
Chloroplast ATP synthase (O1, CF1FO)
Yang, J. (Contributor), Williams, D. (Contributor), Kandiah, E. (Contributor), Fromme, P. (Contributor) & Chiu, P. (Contributor), Protein Data Bank (PDB), Sep 9 2020
DOI: 10.2210/pdb6VOH, https://www.wwpdb.org/pdb?id=pdb_00006voh
Dataset
-
Chloroplast ATP synthase (R2, CF1)
Yang, J. (Contributor), Williams, D. (Contributor), Kandiah, E. (Contributor), Fromme, P. (Contributor) & Chiu, P. (Contributor), Protein Data Bank (PDB), Sep 9 2020
DOI: 10.2210/pdb6VOM, https://www.wwpdb.org/pdb?id=pdb_00006vom
Dataset