Structural and functional analysis of disease‐linked p97 atpase mutant complexes

Purbasha Nandi, Yu Ping Poh, Shan Li, Rod Carlo A. Columbres, Feng Wang, Dewight R. Williams, Po Lin Chiu, Tsui Fen Chou

Research output: Contribution to journalArticlepeer-review

Abstract

IBMPFD/ALS is a genetic disorder caused by a single amino acid mutation on the p97 ATPase, promoting ATPase activity and cofactor dysregulation. The disease mechanism underlying p97 ATPase malfunction remains unclear. To understand how the mutation alters the ATPase reg-ulation, we assembled a full‐length p97R155H with its p47 cofactor and first visualized their structures using single‐particle cryo‐EM. More than one‐third of the population was the dodecameric form. Nucleotide presence dissociates the dodecamer into two hexamers for its highly elevated function. The N‐domains of the p97R155H mutant all show up configurations in ADP‐ or ATPγS‐bound states. Our functional and structural analyses showed that the p47 binding is likely to impact the p97R155H ATPase activities via changing the conformations of arginine fingers. These functional and structural analyses underline the ATPase dysregulation with the miscommunication between the functional modules of the p97R155H.

Original languageEnglish (US)
Article number8079
JournalInternational journal of molecular sciences
Volume22
Issue number15
DOIs
StatePublished - Aug 1 2021
Externally publishedYes

Keywords

  • Arginine finger
  • IBMPFD
  • P47 cofactor
  • P97 ATPase
  • P97 mutation
  • Single‐particle cryo‐EM

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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