Streptococcus mutans serotype c tagatose 6-phosphate pathway gene cluster

E. K. Jagusztyn-Krynicka, J. B. Hansen, V. L. Crow, T. D. Thomas, A. L. Honeyman, R. Curtiss

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

DNA cloned into Escherichia coli K-12 from a serotype c strain of Streptococcus mutans encodes three enzyme activities for galactose utilization via the tagatose 6-phosphate pathway: galactose 6-phosphate isomerase, tagatose 6-phosphate kinase, and tagatose-1,6-bisphosphate aldolase. The genes coding for the tagatose 6-phosphate pathway were located on a 3.28-kb HindIII DNA fragment. Analysis of the tagatose proteins expressed by recombinant plasmids in minicells was used to determine the sizes of the various gene products. Mutagenesis of these plasmids with transposon Tn5 was used to determine the order of the tagatose genes. Tagatose 6-phosphate isomerase appears to be composed of 14- and 19-kDa subunits. The sizes of the kinase and aldolase were found to be 34 and 36 kDa, respectively. These values correspond to those reported previously for the tagatose pathway enzymes in Staphylococcus aureus and Lactococcus lactis.

Original languageEnglish (US)
Pages (from-to)6152-6158
Number of pages7
JournalJournal of Bacteriology
Volume174
Issue number19
StatePublished - 1992
Externally publishedYes

Fingerprint

Streptococcus mutans
Multigene Family
Plasmids
Lactococcus lactis
Fructose-Bisphosphate Aldolase
Gene Order
DNA
Enzymes
Galactose
Recombinant Proteins
Mutagenesis
Genes
Staphylococcus aureus
Phosphotransferases
Escherichia coli
tagatose
tagatose 6-phosphate
Serogroup
galactose-6-phosphate isomerase

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Jagusztyn-Krynicka, E. K., Hansen, J. B., Crow, V. L., Thomas, T. D., Honeyman, A. L., & Curtiss, R. (1992). Streptococcus mutans serotype c tagatose 6-phosphate pathway gene cluster. Journal of Bacteriology, 174(19), 6152-6158.

Streptococcus mutans serotype c tagatose 6-phosphate pathway gene cluster. / Jagusztyn-Krynicka, E. K.; Hansen, J. B.; Crow, V. L.; Thomas, T. D.; Honeyman, A. L.; Curtiss, R.

In: Journal of Bacteriology, Vol. 174, No. 19, 1992, p. 6152-6158.

Research output: Contribution to journalArticle

Jagusztyn-Krynicka, EK, Hansen, JB, Crow, VL, Thomas, TD, Honeyman, AL & Curtiss, R 1992, 'Streptococcus mutans serotype c tagatose 6-phosphate pathway gene cluster', Journal of Bacteriology, vol. 174, no. 19, pp. 6152-6158.
Jagusztyn-Krynicka EK, Hansen JB, Crow VL, Thomas TD, Honeyman AL, Curtiss R. Streptococcus mutans serotype c tagatose 6-phosphate pathway gene cluster. Journal of Bacteriology. 1992;174(19):6152-6158.
Jagusztyn-Krynicka, E. K. ; Hansen, J. B. ; Crow, V. L. ; Thomas, T. D. ; Honeyman, A. L. ; Curtiss, R. / Streptococcus mutans serotype c tagatose 6-phosphate pathway gene cluster. In: Journal of Bacteriology. 1992 ; Vol. 174, No. 19. pp. 6152-6158.
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