Steady-state and time-resolved polarized light spectroscopy of the green plant light-harvesting complex II

S. L.S. Kwa, F. G. Groeneveld, J. P. Dekker, R. van Grondelle, H. van Amerongen, Su Lin, W. S. Struve

Research output: Contribution to journalArticle

Abstract

The major chlorophyll a/b light-harvesting complex from spinach thylakoid membranes was analyzed by steady-state polarized light spectroscopy at 4 K and by one-color and two-color pump-probe spectroscopy at room temperature. Steady-state absorption, linear dichroism and circular dichroism spectra indicate that the Chl Qy(0−0) absorption region is characterized by at least six transitions with significant differences in absorption, orientation and rotational strength. Steady-state low-temperature fluorescence spectra suggest that the fluorescence arises for a large part from several energetically similar species that form a circularly degenerate oscillator in the plane constituted by the two long axes of the particle. The possible presence of special red-absorbing pigments at low temperature is discussed. The time-resolved data suggest that the kinetics of chlorophyllb → a excitation energy transfer, as well as those of downhill excitation transfer among chlorophylla spectral forms, are heterogeneous with both sub-picosecond and picosecond lifetime components.

Original languageEnglish (US)
Pages (from-to)143-146
Number of pages4
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1101
Issue number2
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

    Fingerprint

Keywords

  • A
  • absorbance
  • Antenna
  • CD
  • Chl
  • Chlorophyll
  • chlorophyll
  • circular dichroism
  • LD
  • LHC-II
  • light-harvesting chlorophylla/b complex from Photosystem II
  • linear dichroism
  • Photosystem
  • Photosystem II
  • Polarized light spectroscopy
  • PS
  • Pump-probe spectroscopy

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this