Abstract
The major chlorophyll a/b light-harvesting complex from spinach thylakoid membranes was analyzed by steady-state polarized light spectroscopy at 4 K and by one-color and two-color pump-probe spectroscopy at room temperature. Steady-state absorption, linear dichroism and circular dichroism spectra indicate that the Chl Qy(0-0) absorption region is characterized by at least six transitions with significant differences in absorption, orientation and rotational strength. Steady-state low-temperature fluorescence spectra suggest that the fluorescence arises for a large part from several energetically similar species that form a circularly degenerate oscillator in the plane constituted by the two long axes of the particle. The possible presence of special red-absorbing pigments at low temperature is discussed. The time-resolved data suggest that the kinetics of chlorophyll b → a excitation energy transfer, as well as those of downhill excitation transfer among chlorophyll a spectral forms, are heterogeneous with both sub-picosecond and picosecond lifetime components.
Original language | English (US) |
---|---|
Pages (from-to) | 143-146 |
Number of pages | 4 |
Journal | Biochimica et Biophysica Acta - Bioenergetics |
Volume | 1101 |
Issue number | 2 |
State | Published - 1992 |
Externally published | Yes |
Keywords
- Antenna
- Chlorophyll
- Photosystem II
- Polarized light spectroscopy
- Pump-probe spectroscopy
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Cell Biology